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. 2022 Feb 17;11(2):412.
doi: 10.3390/antiox11020412.

Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity

Adriana Elena Bulboacă  1 , Alina Silvia Porfire  2 , Vasile Rus  3 , Cristina Ariadna Nicula  4 , Corneliu Angelo Bulboacă  5 , Sorana D Bolboacă  6
Affiliations

Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity

Adriana Elena Bulboacă et al. Antioxidants (Basel). .

Abstract

Our study aimed to assess the effect of liposomal epigallocatechin-gallate (LEGCG) compared with epigallocatechin-gallate (EGCG) solution on hepatic toxicity induced by gentamicin (G) administration in rats. Five groups were evaluated, a control group (no G administration) and four groups that received G (1 mL, i.p, 80 mg/kg b.w. (body weight/day), for 7 days) to which we associated daily administration 30 min before G of EGCG (G-EGCG, 2.5 mg/0.1 kg b.w.), LEGCG (G-LEGCG, 2.5 mg/0.1 kg b.w.) or silymarin (100 mg/kg b.w./day). The nitro-oxidative stress (NOx), catalase (CAT), TNF-α, transaminases, creatinine, urea, metalloproteinase (MMP) 2 and 9, and liver histopathological changes were evaluated. LEGCG exhibited better efficacy than EGCG, improving the oxidant/antioxidant balance (p = 0.0125 for NOx and 0.0032 for CAT), TNF-α (p < 0.0001), MMP-2 (p < 0.0001), aminotransferases (p = 0.0001 for AST and 0.0136 for ALT), creatinine (p < 0.0001), urea (p = 0.0006) and histopathologic liver changes induced by gentamicin. Our study demonstrated the beneficial effect of EGCG with superior results of the liposomal formulation for hepatoprotection in experimental hepatic toxicity induced by gentamicin.

Keywords: epigallocatechin gallate (EGCG); gentamicin-induced hepatotoxicity; metalloproteinase (MMP).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Serum levels of TNF-α by groups. C, control group; G, gentamicin group; G-EGCG, gentamicin and epigallocatechin gallate group; G-LEGCG, gentamicin and liposomal epigallocatechin gallate group; G-Sily, gentamicin and silymarin group.
Figure 2
Figure 2
Serum levels of NOx and CAT by groups. C, control group; G, gentamicin group; G-EGCG, gentamicin and epigallocatechin gallate group; G-LEGCG, gentamicin and liposomal epigallocatechin gallate group; G-Sily, gentamicin and silymarin group.
Figure 3
Figure 3
Serum levels of matrix metalloproteinases (MMP) 2 and 9 by groups. C, control group; G, gentamicin group; G-EGCG, gentamicin and epigallocatechin gallate group; G-LEGCG, gentamicin and liposomal epigallocatechin gallate group; G-Sily, gentamicin and silymarin group.
Figure 4
Figure 4
Hepatic lobes; (A)—Control (C) group; (B,C)—gentamicin (G) group; (D)—G-EGCG group; (E)—G-LEGCG group; (F)—G-Sily group, where EGCG = epigallocatechin gallate, LEGCG = liposomal epigallocatechin gallate, Sily = silymarin. The images were taken with a 20× lens and the scale bar is 100 µm. Black arrow—centrilobular vein; red arrow—porto-biliary space; blue arrow—hepatocytes with intensely stained cytoplasm; white arrow—thickened nuclear membrane.
See this image and copyright information in PMC

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