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. 2022 Feb 5;8(2):158.
doi: 10.3390/jof8020158.

Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Fernanda Achimón  1   2 , Vanessa D Brito  1   2 , Romina P Pizzolitto  1   2 , Julio A Zygadlo  1   2   3
Affiliations

Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Fernanda Achimón et al. J Fungi (Basel). .

Abstract

The aim of the present study was to evaluate the effect of different carbon sources on the hydrocarbon-like volatile organic compounds (VOCs) of Fusarium verticillioides strain 7600 through a Principal Component Analysis approach, and to explore their diesel potential by using data from the literature. The fungus was cultivated in GYAM culture medium, and five carbon sources were evaluated: glucose, sucrose, xylose, lactose, and fructose. The VOCs were collected using a close-loop apparatus and identified through GC-MS. The same profile of 81 VOCs was detected with all treatments, but with different relative percentages among carbon sources. The production of branched-chain alkanes (30 compounds) ranged from 25.80% to 38.64%, straight-chain alkanes (12 compounds) from 22.04% to 24.18%, benzene derivatives (12 compounds) from 7.48% to 35.58%, and the biosynthesis of branched-chain alcohols (11 compounds) was from 6.82% to 16.71%, with lower values for the remaining groups of VOCs. Our results show that F. verticillioides has the metabolic potential to synthesize diesel-like VOCs. Further research should include the optimization of culture conditions other than carbon sources to increase the production of certain groups of VOCs.

Keywords: Fusarium verticillioides; carbon sources; mycodiesel; volatile organic compounds.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
PCA of Branched-chain alkanes. A total of 35 variables were included: 30 VOCs (dark grey circles) and 5 carbon sources (light grey circles). The first two principal components accounted for 53.3% and 36.3% of the total variation, respectively.
Figure 2
Figure 2
PCA of Straight-chain alkanes. A total of 17 variables were included: 12 VOCs (dark grey circles) and 5 carbon sources (light grey circles). The first two principal components accounted for 58.0% and 22.1% of the total variation, respectively.
Figure 3
Figure 3
PCA of Benzene derivatives. A total of 17 variables were included: 12 VOCs (dark grey circles) and 5 carbon sources (light grey circles). The first two principal components accounted for 58.9% and 30.4% of the total variation, respectively.
Figure 4
Figure 4
PCA of Straight- and branched-chain alcohols. A total of 18 variables were included: 13 VOCs (dark grey circles) and 5 carbon sources (light grey circles). The first two principal components accounted for 48.0% and 37.5% of the total variation, respectively.
See this image and copyright information in PMC

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