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. 2022 Feb 12;11(4):529.
doi: 10.3390/foods11040529.

Identification of β-Glucosidase Activity of Lentilactobacillus buchneri URN103L and Its Potential to Convert Ginsenoside Rb1 from Panax ginseng

Gereltuya Renchinkhand  1 , Urgamal Magsar  1 , Hyoung Churl Bae  1 , Suk-Ho Choi  2 , Myoung Soo Nam  1
Affiliations

Identification of β-Glucosidase Activity of Lentilactobacillus buchneri URN103L and Its Potential to Convert Ginsenoside Rb1 from Panax ginseng

Gereltuya Renchinkhand et al. Foods. .

Abstract

Lentilactobacillus buchneri isolated from Korean fermented plant foods produces β-glucosidase, which can hydrolyze ginsenoside Rb1 from Panax ginseng to yield ginsenoside Rd. The aim of this study was to determine the mechanisms underlying the extracellular β-glucosidase activity obtained from Lentilactobacillus buchneri URN103L. Among the 17 types of lactic acid bacteria showing positive β-glucosidase activity in the esculin iron agar test, only URN103L was found to exhibit high hydrolytic activity on ginsenoside Rb1. The strain showed 99% homology with Lentilactobacillus buchneri NRRLB 30929, whereby it was named Lentilactobacillus buchneri URN103L. Supernatants of selected cultures with β-glucosidase activity were examined for hydrolysis of the major ginsenoside Rb1 at 40 °C, pH 5.0. Furthermore, the β-glucosidase activity of this strain showed a distinct ability to hydrolyze major ginsenoside Rb1 into minor ginsenosides Rd and Rg3. Lentilactobacillus buchneri URN103L showed higher leucine arylamidase, valine arylamidase, α-galactosidass, β-galactosidase, and β-glucosidase activities than any other strain. We conclude that β-glucosidase from Lentilactobacillus buchneri URN103L can effectively hydrolyze ginsenoside Rb1 into Rd and Rg3. The converted ginsenoside can be used in functional foods, yogurts, beverage products, cosmetics, and other health products.

Keywords: Lentilactobacillus buchneri URN103L; Rd; Rg3; ginsenoside Rb1; hydrolyze; β-glucosidase.

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Conflict of interest statement

The authors declare no conflict of interests.

Figures

Figure 1
Figure 1
Screening of lactic acid bacteria with β-glucosidase activity from fermented plants: (A) single colony of strain URN103L in BCP agar, (B) positive reaction of strain. URN103L in esculin agar.
Figure 2
Figure 2
TLC analysis of ginsenosides hydrolyzed by strain URN103L in Rb1, Rb2, and Re for 7 days. Std: standard.
Figure 3
Figure 3
Phylogenetic tree based on 16S rDNA sequences. Phylogenetic relationships of strain URN103L with other Lentilactobacillus sp. are shown. Bar (0.01) is scale length.
Figure 4
Figure 4
TLC analysis of ginsenoside Rb1 hydrolyzed by crude enzyme of isolated strain URN103L at different pHs (4.0, 5.0, 6.0, 7.0, 8.0, and 9.0) at 35 °C for 7 days.
Figure 5
Figure 5
TLC analysis of ginsenoside Rb1 hydrolyzed by crude enzyme of isolated strain URN103L at different temperatures (30, 35, and 40 °C) at pH 7.0 for 7 days.
Figure 6
Figure 6
TLC analysis of ginsenoside Rb1 conversion by culture broth of Lentilactobacillus buchneri URN103L under the optimum condition for 14 days of hydrolysis. STD: standard.
Figure 7
Figure 7
Conversion of ginsenoside Rb1 by crude enzyme from Lactobacillus buchneri URN103L: (A) 0 days; (B) 3 days; (C) 7 days; (D) 10 days; (E) 14 days.
Figure 8
Figure 8
TLC analysis of ginsenoside root conversion by culture broth of Lentilactobacillus buchneri URN103L at optimum condition. Std: standard.
Figure 9
Figure 9
Ginsenoside conversion of ginseng root by Lentilactobacillus buchineri URN103L for fermentation: (A) Panax ginseng root; (B) 3 days; (C) 7 days; (D) 14 days.
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