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. 2022 Jan 29;12(2):209.
doi: 10.3390/life12020209.

Using Halothermal Time Model to Describe Barley (Hordeumvulgare L.) Seed Germination Response to Water Potential and Temperature

Abd Ullah  1   2   3   4 , Sadaf Sadaf  5 , Sami Ullah  5 , Huda Alshaya  6 , Mohammad K Okla  7 , Yasmeen A Alwasel  7 , Akash Tariq  1   2   3   4
Affiliations

Using Halothermal Time Model to Describe Barley (Hordeumvulgare L.) Seed Germination Response to Water Potential and Temperature

Abd Ullah et al. Life (Basel). .

Abstract

Barley (Hordeum vulgare L.) is a salt-tolerant crop with considerable economic value in salinity-affected arid and semiarid areas. In the laboratory experiment, the halothermal time (HaloTT) model was used to examine barley seed germination (SG) at six constant cardinal temperatures (Ts) of 15, 20, 25, 30, 35, and 40 °C under five different water potentials (ψs) of 0, -0.5, -1.5, -1.0, and -2.0 MPa. Results showed that at optimum moisture (0 MPa), the highest germination percentage (GP) was recorded at 20 °C and the lowest at 40 °C. Moreover, GP increased with the accelerated aging period (AAP) and significantly (p ≤ 0.05) decreased with high T. In addition, with a decrease of ψ from 0 to -0.5, -1, 1.5, and -2.0 MPa, GP decreased by 93.33, 76.67, 46.67, and 33.33%, respectively, in comparison with 0 MPa. The maximum halftime constant (θHalo) and coefficient of determination (R2) values were recorded at 20 °C and 30 °C, respectively. The optimum temperature (To) for barley is 20 °C, base Ψ of 50th percentile (Ψb (50)) is -0.23 Mpa, and standard deviation of Ψb (σΨb) is 0.21 MPa. The cardinal Ts for germination is 15 °C (Tb), 20 °C (To), and 40 °C (Tc). The GP, germination rate index (GRI), germination index (GI), coefficient of the velocity of germination (CVG), germination energy (GE), seed vigor index I and II (SVI-I & II), Timson germination index (GI), and root shoot ratio (RSR) were recorded maximum at 0 MPa at 20 °C and minimum at -2.0 MPa at 40 °C. Mean germination time (MGT) and time to 50% germination (T 50%) were maximum at -2 MPa at 40 °C, and minimum at 20 °C, respectively. In conclusion, the HaloTT model accurately predicted the germination time course of barley in response to T, Ψ, or NaCl. Therefore, barley can be regarded as a salt-tolerant plant and suitable for cultivation in arid and semi-arid regions due to its high resistance to salinity.

Keywords: barley; cardinal temperatures; halothermal time model; seed germination; water potential.

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Conflict of interest statement

Authors have no conflict of interest.

Figures

Figure 1
Figure 1
Cumulative germination for Hordeum vulgare L. at (a) 15 °C, (b) 20 °C, (c) 25 °C, (d) 30 °C, (e) 35 °C, and (f) 40 °C having different water potentials. Symbols indicate water potential and lines indicate cumulative germination.
Figure 1
Figure 1
Cumulative germination for Hordeum vulgare L. at (a) 15 °C, (b) 20 °C, (c) 25 °C, (d) 30 °C, (e) 35 °C, and (f) 40 °C having different water potentials. Symbols indicate water potential and lines indicate cumulative germination.
Figure 2
Figure 2
Plot showing changes in halotime constant (θH) as a function of temperature (T) for Hordeum vulgare L.
Figure 3
Figure 3
Interactive effect of salinity and temperature on (a) germination percentage (b) mean germination time (c) germination rate index (d) germination index of barley (Hordeum vulgare L.) var. AAJ using halo thermal time (HaloTT) model.
Figure 4
Figure 4
Interactive effect of salinity and temperature on (a) coefficient of the velocity of germination (b) germination energy (c) Timson germination index (d) mean germination rate of barley (Hordeum vulgare L.) var. AAJ using HaloTT model.
Figure 5
Figure 5
Interactive effect of salinity and temperature on (a) seed vigor index-I (b) seed vigor index-II (c) time to 50% germination (d) root shoot ratio of barley (Hordeum vulgare L.) var. AAJ using HaloTT model.
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