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. 2022 Jan 26;10(2):290.
doi: 10.3390/microorganisms10020290.

Isolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress Management

Ritu Singh  1   2 , Kapil Deo Pandey  2 , Monika Singh  3 , Sandeep Kumar Singh  2 , Abeer Hashem  4 , Al-Bandari Fahad Al-Arjani  4 , Elsayed Fathi Abd Allah  5 , Prashant Kumar Singh  6 , Ajay Kumar  2
Affiliations

Isolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress Management

Ritu Singh et al. Microorganisms. .

Abstract

In the present study, eight endophytic bacterial strains, namely Bacillus licheniformis R1, Bacillus sp. R2, Agrobacterium tumefaciens R6, uncultured bacterium R11, Bacillus subtilis RS3, Bacillus subtilis RS6, uncultured bacterium RS8 and Lysinibacillus fusiformis RS9, were isolated from the root of Momordica charantia L. All the strains, except R6 exhibited positive for IAA production, siderophore production, and phosphate solubilization during plant growth-promoting traits analysis. Strains invariably utilized glucose and sucrose as a carbon source during substrate utilization, while yeast extract, ammonium sulphate, ammonium chloride, glycine, glutamine, and isoleucine as nitrogen sources. In addition, Spectinomycin was found as the most effective during antibiotic sensitivity TEST, followed by Chloramphenicol, Erythromycin, Rifampicin and Kanamycin, while Polymixin B was found least effective, while strains R1, R6, and RS8 were sensitive to all the antibiotics. Strains R1 and RS6 were able to withstand tolerance up to 10% of NaCl. The strains showing resistance against broad-spectrum antibiotics, especially chloramphenicol, can be used in hospital waste management. In addition, strains with a tolerance of 10 % of NaCl can improve plant growth in the saline affected area.

Keywords: bitter gourd (Momordica charantia L.); endophyte; enzyme activity; stress tolerance; substrate utilization.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The phylogenetic tree of isolated endophytic bacterial strains was constructed using the neighbor-joining method by MEGA 5 software. Each number on a branch indicates the bootstrap confidence values corresponding to the scale bar of branch lengths.
Figure 2
Figure 2
Hierarchical cluster analysis (between groups linkage) measuring the squared Euclidean distance based on their C and N utilization pattern.
Figure 3
Figure 3
Specific growth rate (h−1) of the endophytic isolates grown in LB broth. The values represent ± SE.
Figure 4
Figure 4
Enzymatic activity of endophytic bacterial strains, enzymatic Index represent the halo diameter of degradation/diameter.
See this image and copyright information in PMC

References

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