Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb 11;10(2):418.
doi: 10.3390/microorganisms10020418.

Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling

Thu Huynh  1   2   3 , Mónika Vörös  1 , Orsolya Kedves  1 , Adiyadolgor Turbat  1 , György Sipos  4 , Balázs Leitgeb  5 , László Kredics  1 , Csaba Vágvölgyi  1 , András Szekeres  1
Affiliations

Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling

Thu Huynh et al. Microorganisms. .

Abstract

(1) Background: Bacillus velezensis and Bacillus amyloliquefaciens are closely related members of the "operational group B. amyloliquefaciens", a taxonomical unit above species level within the "Bacillus subtilis species complex". They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the gyrA and rpoB genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish B. velezensis and B. amyloliquefaciens.

Keywords: Bacillus amyloliquefaciens; Bacillus taxonomy; Bacillus velezensis; chemotaxonomy; fatty acid profiling.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Neighbor-Joining phylogenetic tree based on the concatenation of gyrA and rpoB gene sequences. Evolutionary distances were computed by the Tamura-Nei method. Bars, 0.010 substitutions per nucleotide position.
Figure 2
Figure 2
The 2D plot between FA components of B. velezensis and B. amyloliquefaciens. (A) FA components from the group of B. amyloliquefaciens, (B) FA components from the group of B. velezensis.
Figure 3
Figure 3
The relationship of FA profiles among the Bacillus species in the MIS library.
Figure 4
Figure 4
Comparison charts of (A) B. velezensis and (B) B. amiloliqefaciens based on FA profiles created in the MIS library.
See this image and copyright information in PMC

References

    1. Fan B., Blom J., Klenk H.P., Borriss R. Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis form an “Operational Group B. amyloliquefaciens” within the B. subtilis species complex. Front. Microbiol. 2017;8:22. doi: 10.3389/fmicb.2017.00022. - DOI - PMC - PubMed
    1. De Carvalho C.C.C.R., Caramujo M.J. The various roles of fatty acids. Molecules. 2018;23:2583. doi: 10.3390/molecules23102583. - DOI - PMC - PubMed
    1. Choi J., Nam J., Seo M.-H. Complete genome sequence of Bacillus velezensis NST6 and comparison with the species belonging to operational group B. amyloliquefaciens. Genomics. 2021;113:380–386. doi: 10.1016/j.ygeno.2020.12.011. - DOI - PubMed
    1. da Costa M.S., Albuquerque L., Nobre M.F., Wait R. Methods in Microbiology. Volume 38. Elsevier Ltd.; Amsterdam, The Netherlands: 2011. The identification of fatty acids in bacteria; pp. 183–196.
    1. Diomandé S.E., Nguyen-The C., Guinebretière M.H., Broussolle V., Brillard J. Role of fatty acids in Bacillus environmental adaptation. Front. Microbiol. 2015;6:813. doi: 10.3389/fmicb.2015.00813. - DOI - PMC - PubMed

LinkOut - more resources