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. 2022 Feb 16;27(4):1326.
doi: 10.3390/molecules27041326.

Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa

Siddartha Baliyan  1 , Riya Mukherjee  1   2 , Anjali Priyadarshini  1 , Arpana Vibhuti  1 , Archana Gupta  1 , Ramendra Pati Pandey  1 , Chung-Ming Chang  2
Affiliations

Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa

Siddartha Baliyan et al. Molecules. .

Abstract

The use of F. religiosa might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in Ficus religiosa, as F. religiosa is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the measurement of antioxidant properties that includes the use of the free radicals used for assessing the potential of substances to serve as hydrogen providers or free-radical scavengers (FRS). The technique of DPPH testing is associated with the elimination of DPPH, which would be a stabilized free radical. The free-radical DPPH interacts with an odd electron to yield a strong absorbance at 517 nm, i.e., a purple hue. An FRS antioxidant, for example, reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen. It is radical in comparison to the DPPH-H form, because it causes decolorization, or a yellow hue, as the number of electrons absorbed increases. Decolorization affects the lowering capacity significantly. As soon as the DPPH solutions are combined with the hydrogen atom source, the lower state of diphenylpicrylhydrazine is formed, shedding its violet color. To explain the processes behind the DPPH tests, as well as their applicability to Ficus religiosa (F. religiosa) in the manufacture of metal oxide nanoparticles, in particular MgO, and their influence on antioxidants, a specimen from the test was chosen for further study. According to our findings, F. religiosa has antioxidant qualities and may be useful in the treatment of disorders caused by free radicals.

Keywords: 2-diphenyl-1-picrylhydrazyl (DPPH); Ficus religiosa; antioxidants; free-radical scavenging activity; nano-MgO; nanoparticles; nanotechnology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Leaves of F. religiosa and its Crude plant extract.
Figure 2
Figure 2
Antioxidant activity of F. religiosa with DPPH.
Figure 3
Figure 3
Tannin estimation by tannic acid standard.
Figure 4
Figure 4
Standard curve of tannic acid.
Figure 5
Figure 5
Specimen resemblance of total phenolic concentration.
Figure 6
Figure 6
Graph plot of gallic acid results.
Figure 7
Figure 7
Reducing sugar estimation by DNS method.
Figure 8
Figure 8
Specimen resemblance of protein concentration.
Figure 9
Figure 9
Plot Concentration vs. absorbance.
Figure 10
Figure 10
Graph of concentration versus absorbance of blank and standard solution.
See this image and copyright information in PMC

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