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. 2022 Feb 17:15:475-490.
doi: 10.2147/IDR.S350048. eCollection 2022.

Evaluating the Prevalence and the Risk Factors of Gram-Negative Multi-Drug Resistant Bacteria in Eastern Saudi Arabia

Aisha S Al Hamdan  1 , Amal A Alghamdi  2 , Ghada F Alyousif  2 , Fatma A Hamza  2 , Marwa M Shafey  2 , Aisha M AlAmri  3 , Abdulaziz A Sunki  4
Affiliations

Evaluating the Prevalence and the Risk Factors of Gram-Negative Multi-Drug Resistant Bacteria in Eastern Saudi Arabia

Aisha S Al Hamdan et al. Infect Drug Resist. .

Abstract

Purpose: Multidrug-resistant organisms (MDROs) among Gram-negative bacteria (GNB) are a major public health concern worldwide, which can even lead to death. This study was conducted to determine the prevalence of MDROs among isolated GNB in the Security Forces Hospital Dammam (SFHD) and identify its associated risk factors.

Materials and methods: A cross-sectional study was performed on the most commonly isolated GNB in SFHD, Acinetobacter spp., Enterobacter spp., Escherichia coli (E. coli), Klebsiella pneumoniae, Proteus spp., and Pseudomonas aeruginosa, of non-duplicated clinical samples collected from all hospital units throughout the period from January 2017 to December 2018. Data were collected retrospectively from patients' medical records, and analyses were conducted using the chi-square test and logistic regression models.

Results: Of the 1508 GNB included in the study, 969 were multidrug-resistant (MDR; 64.3%). The most commonly identified multidrug-resistant GNB (MDR-GNB) were found in female patients (66.4%) and those aged between 20 and 29 years (21.8%). Urine samples had the highest number of isolated GNB (926 of a total of 1508, 61.4%), and E. coli isolates (53.8%) were the most frequently isolated GNB. Enterobacter spp. had the highest rate of multidrug resistance during the 2 years (64 out of 74, 86.5%). Mechanical ventilation for three or more calendar days was a significant direct risk factor for the development of MDR-GNB (odds ratio [OR]: 2.600, 95% confidence interval [CI]: 1.124-6.012, P = 0.025).

Conclusion: Multidrug resistance is common among GNBs in SFHD. Antimicrobial stewardship programs in hospitals should be supported and implemented. Medical and public awareness of antibiotic use is another significant way to decrease the burden of MDR.

Keywords: antimicrobial resistance; hospital-acquired pathogens; infectious diseases.

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Conflict of interest statement

The authors declare that they have no competing interests in this work.

Figures

Figure 1
Figure 1
Flowchart for study design including inclusion/exclusion criteria of isolates.
Figure 2
Figure 2
[The rate of antibiotics susceptibility among MDR-GNB isolates collected in 2017 AND 2018]. %Susceptibility rate=Number of susceptible isolates/Total number of isolates×100. Boxes in green, yellow and red color reflect susceptibility rate of: 90–100%, 70–89% and <70% respectively.
See this image and copyright information in PMC

References

    1. Blair JM, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJ. Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol. 2015;13(1):42–51. doi:10.1038/nrmicro3380 - DOI - PubMed
    1. Hall KK, Shoemaker-Hunt S, Hoffman L, et al. Making healthcare safer iii: a critical analysis of existing and emerging patient safety practices. Agency for Healthcare Research and Quality (US); 2020. Available from: https://www.ncbi.nlm.nih.gov/books/NBK555533/. Accessed February 4, 2022. - PubMed
    1. Rodríguez-Baño J, Gutiérrez-Gutiérrez B, Machuca I, Pascual A. Treatment of infections caused by extended-spectrum-beta-lactamase-, AmpC-, and carbapenemase-producing Enterobacteriaceae. Clin Microbiol Rev. 2018;31(2). doi:10.1128/CMR.00079-17 - DOI - PMC - PubMed
    1. Antimicrobial resistance: tackling a crisis for the health and wealth of nations. London: Wellcome Trust; 2014.
    1. Dadgostar P. Antimicrobial resistance: implications and costs. Infect Drug Resist. 2019;12:3903–3910. doi:10.2147/IDR.S234610 - DOI - PMC - PubMed

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