doi: 10.1007/978-1-0716-2010-6_17.
Establishment of In Vitro Brain Models for AON Delivery
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- PMID: 35213023
- PMCID: PMC9703237
- DOI: 10.1007/978-1-0716-2010-6_17
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Establishment of In Vitro Brain Models for AON Delivery
Methods Mol Biol.
2022.
Abstract
Progress in stem cell biology has made it possible to generate human-induced pluripotent stem cells (hiPSC) that can be differentiated into complex, three-dimensional structures, where the cells are spatially organized. To study brain development, Lancaster and colleagues developed an hiPSC-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain regions. Here we describe in detail the generation of cerebral organoids using a modified version of the culture protocol.
Keywords: Cerebral organoid; Disease modeling; Induced pluripotent stem cells.
© 2022. The Author(s).
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