. 2022 Feb 21;14(4):908.

doi: 10.3390/nu14040908.

Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model

Laszlo Szabo¹, Richard Molnar¹, Andras Tomesz¹, Arpad Deutsch¹, Richard Darago¹, Timea Varjas¹, Zsombor Ritter², Jozsef L Szentpeteri³, Kitti Andreidesz⁴, Domokos Mathe^{5

6}, Imre Hegedüs⁵, Attila Sik^{3

7}, Ferenc Budan^{1

3

7}, Istvan Kiss¹

Affiliations

¹ Department of Public Health Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
² Department of Medical Imaging, Medical School, University of Pécs, 7624 Pécs, Hungary.
³ Institute of Transdisciplinary Discoveries, Medical School, University of Pécs, 7624 Pécs, Hungary.
⁴ Department of Biochemistry and Medical Chemistry, University of Pécs Medical School, 7624 Pécs, Hungary.
⁵ Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, 1085 Budapest, Hungary.
⁶ Hungarian Centre of Excellence for Molecular Medicine, In Vivo Imaging Advanced Core Facility, 1094 Budapest, Hungary.
⁷ Institute of Physiology, Medical School, University of Pécs, 7624 Pécs, Hungary.

PMID: 35215560
PMCID: PMC8878525
DOI: 10.3390/nu14040908

Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model

Laszlo Szabo et al. Nutrients. 2022.

. 2022 Feb 21;14(4):908.

doi: 10.3390/nu14040908.

Authors

Affiliations

¹ Department of Public Health Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
² Department of Medical Imaging, Medical School, University of Pécs, 7624 Pécs, Hungary.
³ Institute of Transdisciplinary Discoveries, Medical School, University of Pécs, 7624 Pécs, Hungary.
⁴ Department of Biochemistry and Medical Chemistry, University of Pécs Medical School, 7624 Pécs, Hungary.
⁵ Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, 1085 Budapest, Hungary.
⁶ Hungarian Centre of Excellence for Molecular Medicine, In Vivo Imaging Advanced Core Facility, 1094 Budapest, Hungary.
⁷ Institute of Physiology, Medical School, University of Pécs, 7624 Pécs, Hungary.

PMID: 35215560
PMCID: PMC8878525
DOI: 10.3390/nu14040908

Abstract

DNA methylation is an epigenetic mechanism that is crucial for mammalian development and genomic stability. Aberrant DNA methylation changes have been detected not only in malignant tumor tissues; the decrease of global DNA methylation levels is also characteristic for aging. The consumption of extra virgin olive oil (EVOO) as part of a balanced diet shows preventive effects against age-related diseases and cancer. On the other hand, consuming trans fatty acids (TFA) increases the risk of cardiovascular diseases as well as cancer. The aim of the study was to investigate the LINE-1 retrotransposon (L1-RTP) DNA methylation pattern in liver, kidney, and spleen of mice as a marker of genetic instability. For that, mice were fed with EVOO or TFA and were pretreated with environmental carcinogen 7,12-dimethylbenz[a]anthracene (DMBA)-a harmful substance known to cause L1-RTP DNA hypomethylation. Our results show that DMBA and its combination with TFA caused significant L1-RTP DNA hypomethylation compared to the control group via inhibition of DNA methyltransferase (DNMT) enzymes. EVOO had the opposite effect by significantly decreasing DMBA and DMBA + TFA-induced hypomethylation, thereby counteracting their effects.

Keywords: DMBA; LINE-1 methylation pattern; extra virgin olive oil; trans fatty acid.

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Conflict of interest statement

The Authors declare no conflict of interest.

Figures

**Figure 1**
L1-RTP DNA methylation pattern in the spleen of CBA/Ca female mice (n = 6) exposed to the effect of DMBA, and the effect of EVOO or TFA coadministered with DMBA, expressed as the percentage of untreated control (* p < 0.05; *** p < 0.001). L1-RTP DNA: LINE-1 retrotransposon deoxyribonucleic acid, EVOO: extra virgin olive oil, TFA: trans-fatty acid.

**Figure 2**
L1-RTP DNA methylation pattern in the liver of CBA/Ca female mice (n = 6) exposed to the effect of DMBA, and the effect of EVOO or TFA coadministered with DMBA, expressed as the percentage of untreated control (** p < 0.01; *** p < 0.001).

**Figure 3**
L1-RTP DNA methylation pattern in the kidneys of CBA/Ca female mice (n = 6) exposed to the effects of DMBA and the effects of DMBA + EVOO or DMBA + TFA, expressed as the ratio of untreated control (* p < 0.05).

**Figure 4**
Summary of relevant factors influencing inflammation, carcinogenesis DNA methylation, and ageing in connection with our study.

See this image and copyright information in PMC

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Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model

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Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model

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Abstract

Conflict of interest statement

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