Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb 11;14(2):372.
doi: 10.3390/v14020372.

Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine

Najete Safini  1 , Soufiane Elmejdoub  1 , Zahra Bamouh  1 , Mohamed Jazouli  1 , Jihane Hamdi  1 , Zineb Boumart  1 , Halima Rhazi  1 , Khalid Omari Tadlaoui  1 , Mehdi El Harrak  1
Affiliations

Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine

Najete Safini et al. Viruses. .

Abstract

Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia. A combination vaccine to control CBPP and LSD offers significant value to small-scale livestock keepers as a single administration. Access to a bivalent vaccine may improve vaccination rates for both pathogens. In the present study, we evaluated the LSDV/CBPP live combined vaccine by testing the generation of virus neutralizing antibodies, immunogenicity, and safety on target species. In-vitro assessment of the Mycoplasma effect on LSDV growth in cell culture was evaluated by infectious virus titration and qPCR during 3 serial passages, whereas in-vivo interference was assessed through the antibody response to vaccination. This combined Mmm/LSDV vaccine could be used to protect cattle against both diseases with a single vaccination in the endemic countries. There were no adverse reactions detected in this study and inoculated cattle produced high levels of specific antibodies starting from day 7 post-vaccination, suggesting that this combination vaccine is both safe and effective.

Keywords: Lumpy skin disease (LSD); Mycoplasma mycoides subsp. mycoides (Mmm); combined Mmm/LSDVvaccine; contagious bovine pleuropneumonia (CBPP).

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
CPE of LSDV separated and in co-infection with Mmm on LT cell culture (×400). (A) LT infected with Mmm alone. (B) LSDV CPE effect, (C) Mmm/LSDV co-culturing CPE effect on LT cells.
Figure 2
Figure 2
Percentage of seropositive cattle for Mmm and LSDV vaccinated with Mmm and LSDV monovalent vaccines vs. Mmm/LSDV combined vaccines monitored 3 months. D: Days M: Months.
Figure 3
Figure 3
Mean neutralizing antibody titers of seropositive cattle vaccinated with LSDV and Mmm/LSDV vaccines monitored 3 months. Data shown are means of at least three neutralization experiments. D: Days M: Months.
See this image and copyright information in PMC

References

    1. Francis M.I., Oragwa A.O., Ankeli P.I., Liba J.W., Ejeh E.F., Raji M.A., Ameh J.A., Egwu G.O. Prevalence of Contagious Bovine Pleuropneumonia Based on Gross Lesions in Cattle at Slaughter in Adamawa State, Nigeria. Sokoto J. Vet. Sci. 2018;16:31–37. doi: 10.4314/sokjvs.v16i1.5. - DOI
    1. Manso-Silván L., Vilei E.M., Sachse K., Djordjevic S.P., Thiaucourt F., Frey J. Mycoplasma Leachii Sp. Nov. as a New Species Designation for Mycoplasma Sp. Bovine Group 7 of Leach, and Reclassification of Mycoplasma Mycoides Subsp. Mycoides LC as a Serovar of Mycoplasma Mycoides Subsp. Capri. Int. J. Syst. Evol. Microbiol. 2009;59:1353–1358. doi: 10.1099/ijs.0.005546-0. - DOI - PubMed
    1. Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J., Johansson K.E., Pettersson B., Uhlén M. The Genome Sequence of Mycoplasma Mycoides Subsp. Mycoides SC Type Strain PG1T, the Causative Agent of Contagious Bovine Pleuropneumonia (CBPP) Genome Res. 2004;14:221–227. doi: 10.1101/gr.1673304. - DOI - PMC - PubMed
    1. Di Teodoro G., Marruchella G., Di Provvido A., D’Angelo A.R., Orsini G., Di Giuseppe P., Sacchini F., Scacchia M. Contagious Bovine Pleuropneumonia: A Comprehensive Overview. Vet. Pathol. 2020;57:476–489. doi: 10.1177/0300985820921818. - DOI - PubMed
    1. Pilo P., Frey J., Vilei E.M. Molecular Mechanisms of Pathogenicity of Mycoplasma Mycoides Subsp. Mycoides SC. Vet. J. 2007;174:513–521. doi: 10.1016/j.tvjl.2006.10.016. - DOI - PMC - PubMed

Publication types

MeSH terms

Supplementary concepts

LinkOut - more resources