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. 2022:2440:3-39.
doi: 10.1007/978-1-0716-2051-9_1.

Fluorescence Microscopy: A Field Guide for Biologists

Lucy H Swift  1 , Pina Colarusso  2
Affiliations

Fluorescence Microscopy: A Field Guide for Biologists

Lucy H Swift et al. Methods Mol Biol. 2022.

Abstract

Optical microscopy is a tool for observing objects, and features within objects, that are not visible to the unaided eye. In the life sciences, fluorescence microscopy has been widely adopted because it allows us to selectively observe molecules, organelles, and cells at multiple levels of organization. Fluorescence microscopy encompasses numerous techniques and applications that share a specialized technical language and concepts that can create barriers for researchers who are new to this area. Our goal is to meet the needs of researchers new to fluorescence microscopy, by introducing the essential concepts and mindset required to navigate and apply this powerful technology to the laboratory.

Keywords: Data management plan; Digital image; Fluorescence microscopy; Image acquisition; Imaging workflow; Lateral resolution; Magnification; Numerical aperture; Nyquist sampling; Optical microscopy; Resolution.

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