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. 2022:2440:181-196.
doi: 10.1007/978-1-0716-2051-9_11.

Quantifiable Intravital Light Sheet Microscopy

Holly C Gibbs  1   2 , Sreeja Sarasamma  3 , Oscar R Benavides  3 , David G Green  4 , Nathan A Hart  3 , Alvin T Yeh  3 , Kristen C Maitland  3   5 , Arne C Lekven  6
Affiliations

Quantifiable Intravital Light Sheet Microscopy

Holly C Gibbs et al. Methods Mol Biol. 2022.

Abstract

Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB) with light sheet fluorescence microscopy (LSFM) and pilot experiments to create a study-specific set of parameters for semiautomatically tracking cellular movements in the embryonic midbrain primordium during zebrafish segmentation.

Keywords: Bioimage analysis; Light sheet fluorescence microscopy; Midbrain–hindbrain boundary; Zebrafish.

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References

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