Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May;25(5):571-575.
doi: 10.1111/1756-185X.14308. Epub 2022 Feb 26.

Association of Osteopontin gene single nucleotide polymorphism with lupus nephritis

R M Metwally  1 , Ahmad S Hasan  2 , El-Sayed G R  1
Affiliations

Association of Osteopontin gene single nucleotide polymorphism with lupus nephritis

R M Metwally et al. Int J Rheum Dis. 2022 May.

Abstract

Aim: To determine the association of single nucleotide polymorphism at 9250 C/T in exon 7 of the Osteopontin (OPN) gene among Egyptian patients with lupus nephritis (LN) and healthy controls and assess its relation with clinical and laboratory features in addition to both activity and chronicity indices in these patients.

Materials and method: The study population includes 100 patients with LN and 100 age- and gender-matched controls. OPN gene 9250 C/T polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphism.

Results: We observed a significant difference in the frequencies of the OPN gene 9250 T allele between the patients with LN and the controls (74.5% vs 57.5%, P < .001); also, TT and CT + TT genotypes showed significant differences in frequencies between LN patients versus controls (59% vs 35% P = .005 and 90% vs. 80% P = .048, respectively). We also observed a non-significant association between OPN gene 9250 genotypes and each of the laboratory data and clinical features in addition to activity and chronicity indices in all studied LN patients. There were no statistically significant increased TT and CT + TT genotypes and T allele frequencies in LN patients with renal failure compared to those without renal failure. Logistic regression analysis revealed that only OPN (CT + TT) genotype could predict LN development in Egyptian patients.

Conclusion: TT and CT + TT genotypes and T alleles of OPN 9250 are considered risk factors for LN development in Egyptian systemic lupus erythematosus patients. However, these genotypes showed no association with each laboratory data and clinical feature or activity and chronicity indices in these patients. OPN 9250 (CT + TT) genotype could be used to predict LN development in SLE.

Keywords: basic sciences; immunogenetics; systemic lupus erythematous.

PubMed Disclaimer

Comment in

References

REFERENCES

    1. Kotzin BL. Systemic lupus erythematosus. Cell. 1996;85(3):303-306. doi:10.1016/S0092-8674(00)81108-3
    1. Iwata Y, Furuichi K, Kaneko S, Wada T. The role of cytokine in the lupus nephritis. J Biomed Biotechnol. 2011;2011:1-7. doi:10.1155/2011/594809
    1. Wang KX, Denhardt DT. Osteopontin: role in immune regulation and stress responses. Cytokine Growth Factor Rev. 2008;19(5-6):333-345.
    1. Kaleta B. Role of osteopontin in systemic lupus erythematosus. Arch Immunol Ther Exp. 2014;62(6):475-482. doi:10.1007/s00005-014-0294-x
    1. D'Alfonso S, Barizzone N, Giordano M, et al. Two single-nucleotide polymorphisms in the 5' and 3' ends of the osteopontin gene contribute to susceptibility to systemic lupus erythematosus. Arthritis Rheum. 2005;52(2):539-547.

LinkOut - more resources