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Review
. 2022 Feb;76(1):1-5.
doi: 10.18926/AMO/63202.

Mouse Model for Optogenetic Genome Engineering

Tomoka Takao  1 , Daisuke Yamada  1 , Takeshi Takarada  1
Affiliations
Free article
Review

Mouse Model for Optogenetic Genome Engineering

Tomoka Takao et al. Acta Med Okayama. 2022 Feb.
Free article

Abstract

Optogenetics, a technology to manipulate biological phenomena thorough light, has attracted much attention in neuroscience. Recently, the Magnet System, a photo-inducible protein dimerization system which can control the intracellular behavior of various biomolecules with high accuracy using light was developed. Furthermore, photoactivation systems for controlling biological phenomena are being developed by combining this technique with genome-editing technology (CRISPR/Cas9 System) or DNA recombination technology (Cre-loxP system). Herein, we review the history of optogenetics and the latest Magnet System technology and introduce our recently developed photoactivatable Cre knock-in mice with temporal-, spatial-, and cell-specific accuracy.

Keywords: Cre recombinase; optogenetics.

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Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

References

    1. Hegemann P: Algal sensory photoreceptors. Annu Rev Plant Biol (2008) 59: 167-189.
    1. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A and Deisseroth K: Multimodal fast optical interrogation of neural circuitry. Nature (2007) 446: 633-639.
    1. Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, Belfort GM, Lin Y, Monahan PE and Boyden ES: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Nature (2010) 463: 98-102.
    1. Boyden ES, Zhang F, Bamberg E, Nagel G and Deisseroth K: Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci (2005) 8: 1263-1268.
    1. Deisseroth K, Feng G, Majewska AK, Miesenböck G, Ting A and Schnitzer MJ: Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci (2006) 26: 10380- 10386.

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