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. 2022 Jun;74(6):924-931.
doi: 10.1111/lam.13687. Epub 2022 Mar 8.

Impact of electrolyte solution on electrochemical oxidation treatment of Escherichia coli K-12 by boron-doped diamond electrodes

Jakub Budil  1   2 , Ondrej Szabó  1 , Petra Lišková  2 , Pavla Štenclová  1 , Tibor Izsák  1   3 , Štěpán Potocký  1 , Alexander Kromka  1
Affiliations

Impact of electrolyte solution on electrochemical oxidation treatment of Escherichia coli K-12 by boron-doped diamond electrodes

Jakub Budil et al. Lett Appl Microbiol. 2022 Jun.

Abstract

We studied the disinfection efficacy of boron-doped electrodes on Escherichia coli-contaminated water-based solutions in three different electrolytes, physiological solution (NaCl), phosphate buffer (PB), and phosphate buffer saline (PBS). The effect of the electrochemical oxidation treatment on the bacteria viability was studied by drop and spread plate cultivation methods, and supported by optical density measurements. We have found that bacterial suspensions in NaCl and PBS underwent a total inactivation of all viable bacteria within 10 min of the electrochemical treatment. By contrast, experiments performed in the PB showed a relatively minor decrease of viability by two orders of magnitude after 2 h of the treatment, which is almost comparable with the untreated control. The enhanced bacterial inactivation was assigned to reactive chlorine species, capable of penetrating the bacterial cytoplasmic membrane and killing bacteria from within.

Keywords: Escherichia coli; boron-doped diamond; chloride-based electrolyte; chloride-free electrolyte; water disinfection.

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References

    1. Bruguera-Casamada, C., Sirés, I., Prieto, M.J., Brillas, E. and Araujo, R.M. (2016) The ability of electrochemical oxidation with a BDD anode to inactivate Gram-negative and Gram-positive bacteria in low conductivity sulfate medium. Chemosphere 163, 516-524.
    1. Bruguera-Casamada, C., Sirés, I., Brillas, E. and Araujo, R.M. (2017) Effect of electrogenerated hydroxyl radicals, active chlorine and organic matter on the electrochemical inactivation of Pseudomonas aeruginosa using BDD and dimensionally stable anodes. Sep Purif Technol 178, 224-231.
    1. Cano, A., Cañizares, P., Barrera-Díaz, C., Sáez, C. and Rodrigo, M.A. (2012) Use of conductive-diamond electrochemical-oxidation for the disinfection of several actual treated wastewaters. Chem Eng J 211-212, 463-469.
    1. Chaplin, B.P. (2014) Critical review of electrochemical advanced oxidation processes for water treatment applications. Environ Sci Process Impacts 16, 1182-1203.
    1. Cho, M., Kim, J., Kim, J.Y., Yoon, J. and Kim, J.-H. (2010) Mechanisms of Escherichia coli inactivation by several disinfectants. Water Res 44, 3410-3418.