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Comment
. 2022 Mar 3;7(1):68.
doi: 10.1038/s41392-022-00929-9.

Overcoming unintended immunogenicity in immunocompetent mouse models of metastasis: the case of GFP

Christoph Schultheiß  1 , Mascha Binder  2
Affiliations
Comment

Overcoming unintended immunogenicity in immunocompetent mouse models of metastasis: the case of GFP

Christoph Schultheiß et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Unintended immunogenicity of GFP in immunocompetent mouse models of metastasis can be avoided by using models with central tolerance towards GFP. GFP-expressing metastatic 4T1 breast cancer cells form lung metastases after orthotopical transplantation in immunocompromised NOD-SCID mice. In immunocompetent BALB/c mice and a model of peripheral tolerance (Gnrhr-luc/GFP), formation of metastasis is prevented by a GFP-specific CD8+ T cell response independent of GFP expression level. In contrast, GFP-expressing 4T1 cells can form lung metastases while lacking a GFP-directed T cell response in a model of central tolerance towards GFP (Cx3cr1-GFP;CCR2-RFP). Created with BioRender.com
See this image and copyright information in PMC

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References

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