Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Jul;23(1):1-5.
doi: 10.1016/S0006-3495(78)85427-7.

A means for orienting flat cells in flow systems

R T Stovel, R G Sweet, L A Herzenberg

A means for orienting flat cells in flow systems

R T Stovel et al. Biophys J. 1978 Jul.

Abstract

Flattened cells, such as red blood cells, epithelial cells, and sperm of many species, cause problems for fluorescence-activated cell analysis and sorting machines because the flow systems of such devices are unable to control the orientation of these cells as they flow past the detectors. For this reason, the fluorescence or scattered light measurements for identical cells may vary greatly. A flow geometry is here described that orients flat cells in a coaxial flow system so that each cell presents the same aspect to the observation device. A wedge-shaped exit on the sample injection tube in a coaxial flow system is sufficient to produce the desired orientation effect when used with low sample flow rates. Data is presented showing the effect of orientation of fixed chicken erythrocytes on histograms of small forward-angle light-scattering measurements.

PubMed Disclaimer

References

    1. J Histochem Cytochem. 1977 Jul;25(7):763-73 - PubMed
    1. J Histochem Cytochem. 1977 Jul;25(7):870-4 - PubMed
    1. J Histochem Cytochem. 1977 Jul;25(7):857-63 - PubMed
    1. Sci Am. 1976 Mar;234(3):108-17 - PubMed
    1. J Histochem Cytochem. 1977 Jul;25(7):781-3 - PubMed

Publication types