Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jul;251(1 Pt 1):C136-9.
doi: 10.1152/ajpcell.1986.251.1.C136.

Porous-bottom dishes for culture of polarized cells

R E Steele, A S Preston, J P Johnson, J S Handler

Porous-bottom dishes for culture of polarized cells

R E Steele et al. Am J Physiol. 1986 Jul.

Abstract

Porous-bottom dishes offer several advantages for growing and studying epithelia in culture. Many epithelia differentiate more on porous surfaces than on plastic tissue culture dishes. In addition, separate solutions can be maintained on each side of the epithelium and can be sampled easily for studies of transport and other polarized functions. We describe the fabrication of dishes with a cellulose ester filter, a collagen-coated polycarbonate filter, or a collagen membrane forming the surface for cell attachment at the bottom of the dish.

PubMed Disclaimer

LinkOut - more resources