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. 2022 Feb 9:27:100303.
doi: 10.1016/j.jctube.2022.100303. eCollection 2022 May.

Evaluation of TBMDR® and XDRA® for the detection of multidrug resistant and pre-extensively drug resistant tuberculosis

Eunjin Cho  1 , Su Jin Lee  2 , Jiyoung Lim  2 , Dong Sik Kim  2 , Namil Kim  2 , Han Oh Park  2 , Ji-Im Lee  1 , Eunsoon Son  1 , Sang Nae Cho  1 , Wah Wah Aung  3 , Jong Seok Lee  1
Affiliations

Evaluation of TBMDR® and XDRA® for the detection of multidrug resistant and pre-extensively drug resistant tuberculosis

Eunjin Cho et al. J Clin Tuberc Other Mycobact Dis. .

Abstract

This study evaluated the diagnostic performance of the AccuPower® TB&MDR Real-Time PCR (TBMDR®) and AccuPower® XDR-TB Real-Time PCR Kit-A (XDRA®) to detect multidrug-resistant (MDR-TB) and pre-extensively drug-resistant tuberculosis (pre-XDR-TB) in comparison with phenotypic drug susceptibility testing (DST) using MGIT 960 on 234 clinical Mycobacterium tuberculosis isolates. Discrepant results were confirmed by direct-sequencing. Sensitivity and specificity of TBMDR and XDRA for cultured isolates were 81.2% and 95.8% for isoniazid (INH) resistance, 95.7% and 95.7% for rifampicin (RIF) resistance, 84.1% and 99.1% for fluoroquinolone (FQ) resistance, and 67.4% and 100% for second-line injectables resistance. The sensitivities of each drug were equivalent to other molecular DST methods. High concordance was observed when compared to direct-sequencing. We also found that TBMDR and XDRA assays can detect INH, RIF and FQ resistance in isolates with low level resistance-associated mutations which were missed by phenotypic DST. Our study showed TBMDR and XDRA assays could be the useful tools to detect MDR-TB and pre-XDR-TB.

Keywords: Diagnosis; MDR-TB; TBMDR®, XDRA®; pre-XDR-TB.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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References

    1. World Health Organization . WHO; Geneva, Switzerland: 2020. Global tuberculosis report 2020.
    1. World Health Organization. WHO operational handbook on tuberculosis. Module 4: treatment- drug-resistant tuberculosis treatment. Geneva, Switzerland: WHO; 2020. - PubMed
    1. World Health Organization. Rapid communication: key changes to treatment of drug-resistant tuberculosis 2019. Geneva, Switzerland: (WHO/CDS/TB/2019.26).
    1. World Health Organization . World Health Organization; Geneva, Switzerland: 2021. Meeting report of the WHO expert consultation on the definition of extensively drug-resistant tuberculosis, 27–29 October 2020.
    1. World Health Organization . WHO; Geneva, Switzerland: 2018. Technical manual for drug susceptibility testing of medicines used in the treatment of tuberculosis.

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