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. 2022 Mar 4;12(1):3595.
doi: 10.1038/s41598-022-07637-y.

Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat

Kashif Ahmed  1 , Ghulam Shabbir  2 , Mukhtar Ahmed  2 , Sabahat Noor  3 , Atta Mohi Ud Din  2   4 , Maqsood Qamar  3 , Nazia Rehman  3
Affiliations

Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat

Kashif Ahmed et al. Sci Rep. .

Abstract

Drought tolerant germplasm is needed to increase crop production, since water scarcity is a critical bottleneck in crop productivity worldwide. Auxin Regulated Gene involved in Organ Size (ARGOS) is a large protein family of transcription factors that plays a vital role in organ size, plant growth, development, and abiotic stress responses in plants. Although, the ARGOS gene family has been discovered and functionalized in a variety of crop plants, but a comprehensive and systematic investigation of ARGOS genes in locally used commercial wheat cultivars is still yet to be reported. The relative expression of three highly conserved TaARGOS homoeologous genes (TaARGOS-A, TaARGOS-B, TaARGOS-D) was studied in three drought-tolerant (Pakistan-2013, NARC-2009 and NR-499) and three sensitive (Borlaug-2016, NR-514 and NR-516) wheat genotypes under osmotic stress, induced by PEG-6000 at 0 (exogenous control), 2, 4, 6, and 12 h. The normalization of target genes was done using β-actin as endogenous control, whereas DREB3, as a marker gene was also transcribed, reinforcing the prevalence of dehydration in all stress treatments. Real-time quantitative PCR revealed that osmotic stress induced expression of the three TaARGOS transcripts in different wheat seedlings at distinct timepoints. Overall, all genes exhibited significantly higher expression in the drought-tolerant genotypes as compared to the sensitive ones. For instance, the expression profile of TaARGOS-A and TaARGOS-D showed more than threefold increase at 2 h and six to sevenfold increase after 4 h of osmotic stress. However, after 6 h of osmotic stress these genes started to downregulate, and the lowest gene expression was noticed after 12 h of osmotic stress. Among all the homoeologous genes, TaARGOS-D, in particular, had a more significant influence on controlling plant growth and drought tolerance as it showed the highest expression. Altogether, TaARGOSs are involved in seedling establishment and overall plant growth. In addition, the tolerant group of genotypes had a much greater relative fold expression than the sensitive genotypes. Ultimately, Pakistan-2013 showed the highest relative expression of the studied genes than other genotypes which shows its proficiency to mitigate osmotic stress. Therefore, it could be cultivated in arid and semi-arid regions under moisture-deficient regimes. These findings advocated the molecular mechanism and regulatory roles of TaARGOS genes in plant growth and osmotic stress tolerance in contrasting groups of wheat genotypes, accompanied by the genetic nature of identified genotypes in terms of their potential for drought tolerance.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
DREB3 expression levels in drought-tolerant and sensitive wheat genotypes. (Asterisk indicates P < 0.05).
Figure 2
Figure 2
TaARGOS-A expression levels in drought-tolerant and sensitive wheat genotypes. (Asterisk indicates P < 0.05).
Figure 3
Figure 3
TaARGOS-B expression levels in drought-tolerant and sensitive wheat genotypes. (Asterisk indicates P < 0.05).
Figure 4
Figure 4
TaARGOS-D expression levels in drought-tolerant and sensitive wheat genotypes. (Asterisk indicates P < 0.05).
Figure 5
Figure 5
Expression heatmap and cluster analysis for DREB3 and TaARGOS genes among wheat genotypes.
See this image and copyright information in PMC

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