[Differentiation potentials of clonal strains of bone marrow fibroblasts]

Abstract

The clonal nature of CFUf-derived fibroblast colonies was proved by chromosomal analysis of individual colonies and single-colony-derived fibroblast strains using mixed cell cultures from male and female rabbits. CFUf progeny, forming colonies composed of more than 10(3) cells was capable of 20-30 cell doublings during subsequent passages. When transplanted in diffusion chambers, single-colony-derived fibroblast strains formed bone and cartilage simultaneously. Thus, CFUf or part of them can be regarded as bone marrow osteogenic stem cells.