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. 2022 Apr;59(4):1362-1371.
doi: 10.1007/s13197-021-05145-x. Epub 2021 Jun 3.

Effects of caspase activity of yak meat and internal environment changing during aging

Yang Yayuan  1 , Han Ling  1 , Yu Qunli  1 , Gao Yongfang  1 , Shi Hongmei  2
Affiliations

Effects of caspase activity of yak meat and internal environment changing during aging

Yang Yayuan et al. J Food Sci Technol. 2022 Apr.

Abstract

The objective of this study was to investigate the protease family caspases in skeletal muscle and their potential contribution to postmortem proteolysis and meat tenderization. Nine yaks were slaughtered, and samples of Longissimus dorsal were injected with AC-DEVD-CHO at a ratio of 1:1 (w/v) and then stored at 4 °C for 2, 6, 12, 24, 72, and 120 h. Results indicate that the morphological changes of the muscle fibers are significantly obstructed, which is not conducive to the subsequent degradation of proteins. After inhibiting the activity of Caspase- 3, the activity of Caspase-8 and 9 and the energy metabolism was affected. In the case of without inhibition of caspase, the pH value decreased and then increased. The meat color and the water retention are better, the muscle fiber skeleton protein degradation is remarkable, the tenderness is improved. Furthermore, yak meat tenderness was improved by apoptotic pathway during aging.

Keywords: AC-DEVD-CHO; Caspase; Tenderness; Yak.

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Figures

Fig. 1
Fig. 1
Caspase-3, caspase-8 and caspase-9 activities expressed as fold increases from those of the controls. Caspase-3 (a), caspase-8 (b) and caspase-9 (c) activities of yak meat in the two groups stored at 4 °C for 0, 6, 12, 24, 72, and 120 h
Fig.2
Fig.2
The postmortem changes of total protein degradation during muscles of yak meat
Fig. 3
Fig. 3
Shear force in postmortem yak longissimus dorsi muscle. At a specific postmortem time, * indicates significant difference at P < 0.05. n = 6
Fig.4
Fig.4
The Myofibril fragmentation index (MFI) was calculated by multiplyingthe absorbance with 200. MFI of yak meat stored at 4 °C for 2, 6, 12, 24, 72, and 120 h
Fig. 5
Fig. 5
pH values of postmortem yak longissimus dorsi muscle. At a specific postmortem time, * indicates significant difference at P < 0.05. n = 6
Fig. 6
Fig. 6
Nucleotide concentrations in postmortem yak longissimus dorsi muscle. Within a column, means lacking a common superscript letter differ, P < 0.05. n = 6
See this image and copyright information in PMC

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