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. 2022 Mar 7;194(4):252.
doi: 10.1007/s10661-022-09917-6.

Insights to antimicrobial resistance: heavy metals can inhibit antibiotic resistance in bacteria isolated from wastewater

Massab Bashir Khaira  1 , Muhammad Bilal Yusuf  1 , Fariha Khan  2
Affiliations

Insights to antimicrobial resistance: heavy metals can inhibit antibiotic resistance in bacteria isolated from wastewater

Massab Bashir Khaira et al. Environ Monit Assess. .

Abstract

The alarming upsurge in the co-existence of heavy metal and antibiotic resistance may have a devastating impact on humans, animals, and the environment. Four metal-resistant bacteria were isolated from hospital effluents and industrial drain. Heavy metal resistance and antimicrobial resistance were examined in the isolates followed by identification through 16S rRNA gene sequencing. Delftia tsuruhatensis strain FK-01 and Carnobacterium inhibens strain FK-02 tolerated arsenic with maximal tolerated concentration (MTC) of 30 mM and 10 mM, respectively. Staphylococcus hominis strain FK-04 tolerated copper up to 4 mM and lead-resistant Raoultella ornithinolytica strain FK-05 exhibited tolerance to 1 mM lead. The growth kinetics of bacteria were monitored in the presence of metals and the following antibiotics, tetracycline, chloramphenicol, and kanamycin. The presence of arsenate significantly enhanced tetracycline resistance in C. inhibens. Heavy metal-induced antibiotic resistance was also observed in S. hominis and R. ornithinolytica, against chloramphenicol and tetracycline respectively. D. tsuruhatensis showed resistance to kanamycin but when grown in the presence of arsenic and kanamycin, bacteria lost resistance to the antibiotic. Therefore, it is suggested that the novel arsenate-resistant strain Delftia tsuruhatensis FK-01 has a unique ability to inhibit antimicrobial resistance that can be harnessed in bioremediation.

Keywords: Antibiotic resistance; Arsenic; Copper; Heavy metal–resistant bacteria; Lead; Water.

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References

    1. Ahmed, N. H., Baruah, F. K., & Grover, R. K. (2017). Staphylococcus hominis subsp. novobiosepticus, an emerging multidrug-resistant bacterium, as a causative agent of septicaemia in cancer patients. Indian Journal of Medical Research, 146(3), 420–425. https://doi.org/10.4103/ijmr.IJMR_1362_15
    1. Baker-Austin, C., Wright, M. S., Stepanauskas, R., & McArthur, J. V. (2006). Co-selection of antibiotic and metal resistance. Trends in Microbiology, 14(4), 176–182. https://doi.org/10.1016/j.tim.2006.02.006
    1. Ballash, G. A., Albers, A. L., Mollenkopf, D. F., Sechrist, E., Adams, R. J., & Wittum, T. E. (2021). Antimicrobial resistant bacteria recovered from retail ground meat products in the US include a Raoultella ornithinolytica co-harboring blaKPC-2 and blaNDM-5. Scientific Reports, 11(1), 1–12.
    1. Berg, J., Tom-Petersen, A., & Nybroe, O. (2005). Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field. Letters in Applied Microbiology, 40(2), 146–151. https://doi.org/10.1111/j.1472-765X.2004.01650.x
    1. Biswas, R., Halder, U., Kabiraj, A., Mondal, A., & Bandopadhyay, R. (2021). Overview on the role of heavy metals tolerance on developing antibiotic resistance in both Gram-negative and Gram-positive bacteria. Archives of Microbiology, 203, 2761–2770.

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