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. 2022 Feb 21:13:794950.
doi: 10.3389/fmicb.2022.794950. eCollection 2022.

Arsenic Transformation in Soil-Rice System Affected by Iron-Oxidizing Strain (Ochrobactrum sp.) and Related Soil Metabolomics Analysis

Ziyan Qian  1   2 , Chuan Wu  2   3 , Weisong Pan  1 , Xiaoran Xiong  1 , Libing Xia  1 , Waichin Li  3
Affiliations

Arsenic Transformation in Soil-Rice System Affected by Iron-Oxidizing Strain (Ochrobactrum sp.) and Related Soil Metabolomics Analysis

Ziyan Qian et al. Front Microbiol. .

Abstract

Iron-oxidizing bacteria (FeOB) could oxidize Fe(II) and mediate biomineralization, which provides the possibility for its potential application in arsenic (As) remediation. In the present study, a strain named Ochrobactrum EEELCW01 isolated previously, was inoculated into paddy soils to investigate the effect of FeOB inoculation on the As migration and transformation in paddy soils. The results showed that inoculation of Ochrobactrum sp. increased the proportion of As in iron-aluminum oxide binding fraction, which reduced the As bioavailability in paddy soils and effectively reduced the As accumulation in rice tissues. Moreover, the inoculation of iron oxidizing bacteria increased the abundance of KD4-96, Pedosphaeraceae and other bacteria in the soils, which could reduce the As toxicity in the soil through biotransformation. The abundance of metabolites such as carnosine, MG (0:0/14:0/0:0) and pantetheine 4'-phosphate increased in rhizosphere soils inoculated with FeOB, which indicated that the defense ability of soil-microorganism-plant system against peroxidation caused by As was enhanced. This study proved that FeOB have the potential application in remediation of As pollution in paddy soil, FeOB promotes the formation of iron oxide in paddy soil, and then adsorbed and coprecipitated with arsenic. On the other hand, the inoculation of Ochrobactrum sp. change soil microbial community structure and soil metabolism, increase the abundance of FeOB in soil, promote the biotransformation process of As in soil, and enhance the resistance of soil to peroxide pollution (As pollution).

Keywords: arsenic; iron-oxidizing bacteria; metabolomics; microbial community; paddy soil.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Available arsenic content in soils in different treatments at different growth stages.
FIGURE 2
FIGURE 2
Relative distribution of different arsenic binding states of different treatment groups at the maturing stage in paddy soils.
FIGURE 3
FIGURE 3
Contents of arsenic in different speciation in roots (A) and stems (B) of rice plants.
FIGURE 4
FIGURE 4
Venn diagram of the microbial community at the OUT (A) and genus (B) level.
FIGURE 5
FIGURE 5
Circos diagram of microbial on phylum levels in different treatments.
FIGURE 6
FIGURE 6
Heat map of microbial communities at the genus level.
FIGURE 7
FIGURE 7
PCA scoring model of different treatments.
FIGURE 8
FIGURE 8
PLS-DA scoring [(A) CK and FB and (B) RP and RF] models and permutation test [(C) CK and FB and (D) RP and RF].
FIGURE 9
FIGURE 9
Volcano map of metabolic differences (A: CK vs. FB; and B: RP vs. RF).
FIGURE 10
FIGURE 10
Heat map of metabolic differences of the top 30 metabolites in unplanted (A) and rice rhizosphere (B) soils.
See this image and copyright information in PMC

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