Role of lipopolysaccharide and capsule in the serum resistance of bacteremic strains of Escherichia coli

Abstract

To define the relative roles of capsule and lipopolysaccharide in the virulence of Escherichia coli obtained from blood, we compared the behavior of K1- and K5-encapsulated strains in serum bactericidal and rat virulence assays. Unencapsulated isogenic mutants selected from five parent strains of E. coli O12:K1, but not of O18:K1 or O7:K1 (all rough-specific phage insensitive), were lysed by normal human sera. In contrast, isogenic mutants from strains of serotypes O6:K5 and O18:K5 retained the serum resistance of the parent strains. There was a greater than 10(5) difference in LD50 in newborn rats between K1-positive and K1-negative pairs of E. coli serotypes O18 and O7 and a greater than 1 log difference between isogenic pairs of serotype O12; however, the K5 isogenic pairs had a similar LD50. Some non-O6 O serotypes, however, required the K5 capsule for serum resistance. We conclude that some O serotypes require encapsulation for optimal virulence but that other O serotypes may not.