Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar;24(1):226-242.
doi: 10.1007/s10126-022-10102-6. Epub 2022 Mar 9.

The Genome of the Marine Rotifer Brachionus manjavacas: Genome-Wide Identification of 310 G Protein-Coupled Receptor (GPCR) Genes

Duck-Hyun Kim  1 , Eunjin Byeon  1 , Min-Sub Kim  1 , Young Hwan Lee  1 , Jun Chul Park  2 , Atsushi Hagiwara  3   4 , Jae-Seong Lee  5
Affiliations

The Genome of the Marine Rotifer Brachionus manjavacas: Genome-Wide Identification of 310 G Protein-Coupled Receptor (GPCR) Genes

Duck-Hyun Kim et al. Mar Biotechnol (NY). 2022 Mar.

Abstract

The marine rotifer Brachionus manjavacas is widely used in ecological, ecotoxicological, and ecophysiological studies. The reference genome of B. manjavacas is a good starting point to uncover the potential molecular mechanisms of responses to various environmental stressors. In this study, we assembled the whole-genome sequence (114.1 Mb total, N50 = 6.36 Mb) of B. manjavacas, consisting of 61 contigs with 18,527 annotated genes. To elucidate the potential ligand-receptor signaling pathways in marine Brachionus rotifers in response to environmental signals, we identified 310 G protein-coupled receptor (GPCR) genes in the B. manjavacas genome after comparing them with three other species, including the minute rotifer Proales similis, Drosophila melanogaster, and humans (Homo sapiens). The 310 full-length GPCR genes were categorized into five distinct classes: A (262), B (26), C (7), F (2), and other (13). Most GPCR gene families showed sporadic evolutionary processes, but some classes were highly conserved between species as shown in the minute rotifer P. similis. Overall, these results provide potential clues for in silico analysis of GPCR-based signaling pathways in the marine rotifer B. manjavacas and will expand our knowledge of ligand-receptor signaling pathways in response to various environmental signals in rotifers.

Keywords: Brachionus manjavacas; G protein-coupled receptor; Genome; Marine rotifer.

PubMed Disclaimer

References

    1. Abrusán G, Grundmann N, DeMester L, Makalowski W (2009) TEclass-a tool for automated classification of unknown eukaryotic transposable elements. Bioinformatics 25:1329–1330 - PubMed
    1. Alexander SPH, Kelly E, Mathie A, Peters JA, Veale EL, Armstrong JF, Faccenda E, Harding SD, Pawson AJ, Sharman JL, Southan C, Buneman OP, Cidlowski JA, Christopoulos A, Davenport AP, Fabbro D, Spedding M, Striessnig J, Davies JA, Collaborators CGTP (2019) The concise guide to pharmacology 2019/20: Introduction and other protein targets. Br J Pharmacol 176:S1–S20 - DOI - PubMed - PMC
    1. Bjarnadóttir TK, Gloriam DE, Hellstrand SH, Kristiansson H, Fredriksson R, Schiöth HB (2006) Comprehensive repertoire and phylogenetic analysis of the G protein-coupled receptors in human and mouse. Genomics 88:263–273 - PubMed
    1. Bock MJ, Jarvis GC, Corey EL, Stone EE, Gribble KE (2019) Matenal age alters offspring lifespan, fitness, and lifespan extension under caloric restriction. Sci Rep 9:3138 - PubMed - PMC
    1. Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120 - PubMed - PMC

Substances

LinkOut - more resources