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. 2022 Mar 9;12(1):3878.
doi: 10.1038/s41598-022-07845-6.

High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk

Sima Hassani  1 , Mir-Hassan Moosavy  2 , Sahar Nouri Gharajalar  3 , Seyed Amin Khatibi  1 , Abolfazl Hajibemani  4 , Zahra Barabadi  5
Affiliations

High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk

Sima Hassani et al. Sci Rep. .

Abstract

This study aimed to investigate the prevalence of foodborne pathogenic bacteria in bovine milk, their antibiogram phenotype, and the carriage of antibiotic resistance genes. Raw bovine milk samples (n = 100) were randomly collected from different suppliers in the northwest of Iran. Antibiotic-resistant patterns and the presence of antibiotic resistance genes were evaluated in the isolates. Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. were isolated from 78%, 47%, 25%, and 21% of samples, respectively. All isolates showed high rates of resistance to amoxicillin, penicillin, and cefalexin. The blaTEM and blaSHV genes were detected in 50.0% and 6.4% of E. coli isolates, respectively. Also, 28.5% and 19.0% of Salmonella isolates were positive for blaTEM and blaSHV. The frequency of mecA and blaZ in S. aureus isolates was 20.0% and 12.0%, respectively. The high prevalence of bovine milk contamination with antimicrobial-resistant species in this study necessitates precise control on antibiotic prescription in veterinary medicine.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Electrophoresis pattern obtained by multiplex PCR for detection of blaTEM and blaSHV genes in E. coli isolates. M: marker 100 bp; lane 1, 2, 3, 4, 5, 6, 7, 8, 9, 11: amplification of blaTEM gene at 516 bp; lane 6, 10: amplification of blaSHV gene at 392 bp.
Figure 2
Figure 2
Electrophoresis pattern obtained by multiplex PCR for detection of blaTEM and blaSHV genes in Salmonella isolates. M: marker 100 bp; Lanes 2, 5, 6, 8, and 10: amplification of blaTEM gene at 516 bp; lane 2, 3, 6: amplification of blaSHV gene at 392 bp.
Figure 3
Figure 3
Electrophoresis pattern obtained by multiplex PCR for detection of mecA and blaZ genes in S. aureus isolates. M: marker 100 bp; Lanes 5 and 9: amplification of mecA gene at 532 bp; lane 10: amplification of blaZgene at 700 bp.
See this image and copyright information in PMC

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