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. 2022 Feb 21:13:827668.
doi: 10.3389/fphar.2022.827668. eCollection 2022.

Pharmacokinetics, Tissue Distribution, and Excretion Characteristics of a Radix Polygoni Multiflori Extract in Rats

Wenhao Cheng  1   2 , Siyang Wu  2 , Zheng Yuan  2 , Weiyu Hu  3 , Xin Yu  2 , Nianxin Kang  4 , Qiutao Wang  5 , Mingying Zhu  2 , Kexin Xia  2 , Wei Yang  2 , Chen Kang  2 , Shuofeng Zhang  1 , Yingfei Li  2
Affiliations

Pharmacokinetics, Tissue Distribution, and Excretion Characteristics of a Radix Polygoni Multiflori Extract in Rats

Wenhao Cheng et al. Front Pharmacol. .

Abstract

Although progress has been achieved in the pharmacological activity and toxicity of Radix Polygoni Multiflori (RPM), the chemical basis of its toxicity is still unclear. Here, we performed a multicompound pharmacokinetic analysis and investigated the tissue distribution and excretion characteristics of RPM components after oral administration in rats. The findings demonstrated that the active ingredients of the RPM extract were quickly absorbed after oral administration, with high exposure levels of emodin, 2,3,5,4'-teterahydroxystilbene-2-O-β-D-glucoside (TSG), citreorosein, torachrysone-8-O-glucoside (TG), emodin-8-O-β-D-glucoside (EG), and physcion-8-O-β-D-glucoside (PG). The tissue distributions of emodin, TSG, TG, EG, and PG were high in the liver and kidney. These components were the key contributors to the effectiveness and toxicity of RPM on the liver and kidney. Most of the active ingredients were mainly excreted through feces and bile, while a few were converted into other products in the body and excreted through urine and feces.

Keywords: Radix Polygoni Multiflori; UPLC-MS/MS; excretion; pharmacokinetics; tissue distribution.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The structures of the 13 constituents of RPM and intenal standard.
FIGURE 2
FIGURE 2
Mean plasma concentration-time profiles of the 10 constituents after administration of RPM (6, 18 and 36 g/kg) to rats. The upper error bars represent the standard deviation obtained from six replicates.
FIGURE 3
FIGURE 3
Tissue distribution of the RPM constituents in different sampling time after rat orally administration of RPM extract (18 g/kg).
FIGURE 4
FIGURE 4
The mean cumulative urinary and biliary excretion profile of the eight constituents in rat after single p.o. administration of RPM extract at 18 g/kg. The upper error bars represent the standard deviation obtained from six replicates.
FIGURE 5
FIGURE 5
The mean cumulative fecal excretion profile of the eight constituents in rat after single p.o. administration of RPM extract at 18 g/kg. The upper error bars represent the standard deviation obtained from six replicates.
See this image and copyright information in PMC

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