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. 2022 Feb 28;27(5):1602.
doi: 10.3390/molecules27051602.

Assessment of Antioxidant and Anticancer Activities of Microgreen Alga Chlorella vulgaris and Its Blend with Different Vitamins

Ragaa A Hamouda  1   2 , Amera Abd El Latif  3 , Ebtihal M Elkaw  2 , Amenah S Alotaibi  4 , Asma Massad Alenzi  4 , Hanafy A Hamza  2
Affiliations

Assessment of Antioxidant and Anticancer Activities of Microgreen Alga Chlorella vulgaris and Its Blend with Different Vitamins

Ragaa A Hamouda et al. Molecules. .

Abstract

There is a very vital antioxidant extracted from microgreen alga. Chlorella vulgaris has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), and Ascorbic acid (c) at concentrations of 0.02, 0.04, 0.06, and 0.08 mg/L of each. Each vitamin was added to the BG11 growth medium to determine the effect on growth, total carbohydrate, total protein, pigments content, antioxidant activities of Chlorella vulgaris. Moreover, antitumor effects of methanol extract of C. vulgaris without and with the supplement of thiamine against Human prostate cancer (PC-3), Hepatocellular carcinoma (HEPG-2), Colorectal carcinoma (HCT-116) and Epitheliod Carcinoma (Hela) was estimated in vitro. C. vulgaris supplemented with various vitamins showed a significant increase in biomass, pigment content, total protein, and total carbohydrates in comparison to the control. Thiamine was the best vitamin influencing as an antioxidant. C. vulgaris supplemented with thiamine had high antitumor effects in vitro. So, it's necessary to add vitamins to BG11 media for enhancement of the growth and metabolites.

Keywords: Chlorella vulgaris; antioxidant; antitumor; carbohydrate; vitamins.

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Conflict of interest statement

The authors declare that they have no known competing interest.

Figures

Figure 1
Figure 1
Effect of different concentrations of Pyridoxal (vitamin B6) (a), Ascorbic acids (vitamin C) (b), Thiamine (vitamin B1) (c), and riboflavin (vitamin B2) (d) on C. vulgaris growth measured by Optical density (nm).
Figure 2
Figure 2
Effects of different concentrations of the tested vitamins supplementation on antioxidant activities of C. vulgaris as determined by DPPH (Antioxidant assay) 1,1-Diphenyl-2-picryl-hydrazyl.
Figure 3
Figure 3
The FT-IR frequency range and the following functional groups are present in the C. vulgaris and with thiamine.
See this image and copyright information in PMC

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