Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb 22;11(5):583.
doi: 10.3390/plants11050583.

Brocchia cinerea (Delile) Vis. Essential Oil Antimicrobial Activity and Crop Protection against Cowpea Weevil Callosobruchus maculatus (Fab.)

Abdelkrim Agour  1 , Ibrahim Mssillou  1 , Hamza Mechchate  2 , Imane Es-Safi  2 , Aimad Allali  3 , Azeddin El Barnossi  4 , Omkulthom Al Kamaly  5 , Samar Zuhair Alshawwa  5 , Abdelfattah El Moussaoui  4 , Amina Bari  4 , Badiaa Lyoussi  1 , Elhoussine Derwich  1
Affiliations

Brocchia cinerea (Delile) Vis. Essential Oil Antimicrobial Activity and Crop Protection against Cowpea Weevil Callosobruchus maculatus (Fab.)

Abdelkrim Agour et al. Plants (Basel). .

Abstract

Antibiotics and synthetic pesticides are now playing a role in the spread of resistant pathogens. They continue to have negative consequences for animal and plant health. The goal of this work is to identify the chemical composition of Brocchia cinerea (Delile) Vis. essential oil (EO) using GC-MS(Gas Chromatography-Mass Spectrometer), evaluate its antimicrobial properties, and investigate its insecticidal and repellent effectiveness against Callosobruchus maculatus (C. maculatus). The GC-MS indicated the presence of 21 chemicals, with thujone (24.9%), lyratyl acetate (24.32%), camphor (13.55%), and 1,8-cineole (10.81%) being the most prominent. For the antimicrobial assay, the yeast Candida albicans was very sensitive to the EO with a growth inhibition diameter of (42.33 mm), followed by Staphylococcus aureus (31.33 mm). Fusarium oxysporum is the mycelia strain that appeared to be extremely sensitive to the utilized EO (88.44%) compared to the two species of Aspergillus (A. flavus (48.44%); A. niger (36.55%)). The results obtained in the microdilution method show that Pseudomonas aeruginosa was very sensitive to the EO, inhibited by a very low dose (0.0018 mg/mL). The minimum inhibitory concentration (MIC) results were between 0.0149 and 0.06 mg/mL. B. cinerea EO also demonstrated a potent insecticidal effect and a medium repulsive effect against C. maculatus. Thus, the LC50 value in the contact test was 0.61 μL/L of air, lower than that observed in the inhalation test (0.72 μL/L of air). The present study reveals that B. cinerea EO has the potential to be an antimicrobial and insecticidal agent with a better performance against several pathogenic microorganisms.

Keywords: Brocchia cinerea; Callosobruchus maculatus; antimicrobial activity; cowpea; essential oil; insecticidal; pest control.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 2
Figure 2
The effects of inhaling essential oil of B. cinerea (EOBC) on the mortality of adults of C. maculatus.
Figure 3
Figure 3
The effects of B. cinerea essential oil on the mortality of adults of C. maculatus by contact.
Figure 1
Figure 1
Chromatogram of B. cinerea essential oils GC-MS.
See this image and copyright information in PMC

References

    1. El-Saber Batiha G., Hussein D.E., Algammal A.M., George T.T., Jeandet P., Al-Snafi A.E., Tiwari A., Pagnossa J.P., Lima C.M., Thorat N.D., et al. Application of Natural Antimicrobials in Food Preservation: Recent Views. Food Control. 2021;126:108066. doi: 10.1016/j.foodcont.2021.108066. - DOI
    1. Akromah Z.N., Jayanegara A. Meta-Analysis of Essential Oil Effectiveness against Phythopathogen in Forest Plant Nurseries. IOP Conf. Ser. Earth Environ. Sci. 2021;667:012005. doi: 10.1088/1755-1315/667/1/012005. - DOI
    1. Xie F., Rizvi S.A.H., Zeng X., Feng X., Syed A.H.R., Xinnian Z. Fumigant Toxicity and Biochemical Properties of (α + β) Thujone and 1,8-Cineole Derived from Seriphidium Brevifolium Volatile Oil against the Red Imported Fire Ant Solenopsis Invicta (Hymenoptera: Formicidae) Rev. Bras. Farm. 2019;29:720–727. doi: 10.1016/j.bjp.2019.04.013. - DOI
    1. Tozlu E., Cakir A., Kordali S., Tozlu G., Ozer H., Akcin T.A. Chemical Compositions and Insecticidal Effects of Essential Oils Isolated from Achillea Gypsicola, Satureja Hortensis, Origanum Acutidens and Hypericum Scabrum against Broadbean Weevil (Bruchus Dentipes) Sci. Hortic. 2011;130:9–17. doi: 10.1016/j.scienta.2011.06.019. - DOI
    1. Zimmermann R.C., Aragão C.E.d.C., de Araújo P.J.P., Benatto A., Chaaban A., Martins C.E.N., Amaral W.D., Cipriano R.R., Zawadneak M.A. Insecticide Activity and Toxicity of Essential Oils against Two Stored-Product Insects. Crop Prot. 2021;144:105575. doi: 10.1016/j.cropro.2021.105575. - DOI

LinkOut - more resources