Ultrasensitive colorimetric detection of amoxicillin based on Tris-HCl-induced aggregation of gold nanoparticles

Abstract

An ultrasensitive colorimetric aptasensor for the detection of amoxicillin (AMO) based on the Tris-HCl buffer-induced aggregation of gold nanoparticles (AuNPs) was developed. The AuNPs were aggregated by the addition of Tris-HCl buffer. The adsorption of the aptamer on the AuNP surface increased its negative charge density, leading to the enhancement of the electrostatic repulsion between the nanoparticles, thus protecting AuNPs from aggregation in the Tris-HCl buffer. However, the specific binding of the aptamer with AMO induced conformational changes in the aptamer, which reduced the adsorption of the aptamer on the AuNP surface, diminishing the protective effect of the aptamer. This resulted in the aggregation of AuNPs by Tris-HCl buffer, and consequently, color change of the solution containing AuNPs from red to blue. Under optimized conditions, a linear relationship between the absorbance ratio variation (ΔA₆₈₀/A₅₂₀) and the AMO concentration was observed in the concentration range of 0.1-125 nM, with a detection limit of 67 pM. The developed biosensor exhibited high selectivity toward AMO. Moreover, this strategy was successfully applied to the detection of AMO in lake water samples. Thus, the present aptasensor is a promising alternative for the simple and ultrasensitive detection of AMO in the environment.

Keywords: Amoxicillin; Colorimetric aptasensors; DNA aptamer; Gold nanoparticles; UV–Vis absorbance.