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. 2022 Mar 10;12(1):3940.
doi: 10.1038/s41598-022-07753-9.

Response of N2O emission and denitrification genes to different inorganic and organic amendments

Yajun Yang  1   2 , Hexiang Liu  1   2 , Jialong Lv  3   4
Affiliations

Response of N2O emission and denitrification genes to different inorganic and organic amendments

Yajun Yang et al. Sci Rep. .

Abstract

Denitrification is a key biochemical process in nitrogen cycling and nitrous oxide (N2O) production. In this study, the impacts of different inorganic and organic amendments (OAs) on the abundance of denitrifying genes (nirS, nirK and nosZ) and the level of N2O emission were examined with incubation experiments. Six treatments included the indicated applications: (i) no fertilization (CK); (ii) urea application alone (U); (iii) wheat straw plus urea (U + WS); (iv) pig manure plus urea (U + PM); (v) compost product plus urea (U + CP); and (vi) improved compost product plus urea (U + IC). The results indicated that all fertilization treatments increased accumulative N2O emissions compared with the CK treatment. The U + WS, U + PM and U + CP treatments increased N2O emissions by 2.12-141.3%, and the U + IC treatment decreased N2O emissions by 23.24% relative to the U treatment. nirK was the dominant denitrification gene rather than nirS and nosZ found in soil. Additionally, the highest abundance of nirK gene was that with the U + PM treatment, and the lowest was that with the U + IC treatment. Additionally, changes in the nirK gene were highly correlated with levels of dissolved organic carbon (DOC), dissolved organic nitrogen (DON) and nitrate nitrogen (NO3-N). Automatic linear modeling revealed that N2O emission was closely related to the nirK gene, DOC and NO3-N. Overall, the use of urea and improved compost as co-amendments retarded N2O emission to a considerable degree compared with other OA additions.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
The changes of N2O emission (a) and cumulative N2O emission (b) during the incubation affected by different OAs. CK no chemical fertilizer, U urea, U + WS urea plus wheat straw, U + PM urea plus pig manure, U + CP urea plus compost, U + IC urea plus improved compost.
Figure 2
Figure 2
Relative abundance of denitrification genes during the incubation affected by different OAs. Error bars indicate standard deviations (n = 3). The different letters indicate significant differences (P < 0.05) (nirS (a); nirK (b); nosZ (c); CK no urea and OAs, U urea, U + WS urea plus wheat straw, U + PM urea plus pig manure, U + CP urea plus compost, U + IC urea plus improved compost).
Figure 3
Figure 3
Principal component analysis (PCA) of soil properties, cumulative N2O emission and abundance of denitrification genes (a); predictive importance of denitrifier gens abundance, and soil properties on cumulative N2O emission as determined by automatic linear modeling (b) (DOC dissolved organic carbon, MBN microbial biomass carbon, DON dissolved organic nitrogen, SOC soil organic carbon, CK no urea and OAs, U urea, U + WS urea plus wheat straw, U + PM urea plus pig manure, U + CP urea plus compost, U + IC urea plus improved compost).
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References

    1. IPCC . Climate change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press; 2013.
    1. Pachauri RK, Allen MR, Barros VR, Broome J, Cramer W, Christ R, Church JA, Clarke L, Dahe Q, Dasgupta P. Climate change 2014: Synthesis report. Contribution of Working Groups I, II and III to the fifth assessment report of the Intergovernmental Panel on Climate Change. IPCC; 2014.
    1. Reay DS, Davidson EA, Smith KA, Smith P, Melillo JM, Dentener F, Crutzen PJ. Global agriculture and nitrous oxide emissions. Nat. Clim. Change. 2012;2:410–416.
    1. Jassal RS, Black TA, Roy R, Ethier G. Effect of nitrogen fertilization on soil CH4 and N2O fluxes, and soil and bole respiration. Geoderma. 2011;162:182–186.
    1. Hu HW, Chen D, He JZ. Microbial regulation of terrestrial nitrous oxide formation: Understanding the biological pathways for prediction of emission rates. FEMS Microbiol. Rev. 2015;39:729–749. - PubMed

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