Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May;34(5):e14357.
doi: 10.1111/nmo.14357. Epub 2022 Mar 13.

Tamoxifen administration alters gastrointestinal motility in mice

Sukhada Bhave  1 , Wing Lam N Ho  1 , Katarina Cheng  1 , Meredith Omer  1 , Nicole Bousquet  1 , Richard A Guyer  1 , Ryo Hotta  1 , Allan M Goldstein  1
Affiliations

Tamoxifen administration alters gastrointestinal motility in mice

Sukhada Bhave et al. Neurogastroenterol Motil. 2022 May.

Abstract

Background: Tamoxifen is widely used for Cre-estrogen receptor-mediated genomic recombination in transgenic mouse models to mark cells for lineage tracing and to study gene function. However, recent studies have highlighted off-target effects of tamoxifen in various tissues and cell types when used for induction of Cre recombination. Despite the widespread use of these transgenic Cre models to assess gastrointestinal (GI) function, the effect of tamoxifen exposure on GI motility has not been described.

Methods: We examined the effects of tamoxifen on GI motility by measuring total GI transit, gastric emptying, small intestinal transit, and colonic contractility in wild-type adult mice.

Key results: We observed a significant delay in total GI transit in tamoxifen-treated mice, with unaltered gastric emptying, accelerated small intestinal transit, and abnormal colonic motility.

Conclusion: Our findings highlight the importance of considering GI motility alterations induced by tamoxifen when designing protocols that utilize tamoxifen as a Cre-driver for studying GI function.

Keywords: Cre-ER models; gastrointestinal motility; tamoxifen.

PubMed Disclaimer

References

REFERENCES

    1. Barakat R, Oakley O, Kim H, Jin J, Ko CJ. Extra-gonadal sites of estrogen biosynthesis and function. BMB Rep. 2016;49:488-496. doi:10.5483/bmbrep.2016.49.9.141
    1. Shehata M, van Amerongen R, Zeeman AL, Giraddi RR, Stingl J. The influence of tamoxifen on normal mouse mammary gland homeostasis. Breast Cancer Res. 2014;16:411. doi:10.1186/s13058-014-0411-0
    1. Ahn SH, Granger A, Rankin MM, Lam CJ, Cox AR, Kushner JA. Tamoxifen suppresses pancreatic beta-cell proliferation in mice. PLoS One. 2019;14:e0214829. doi:10.1371/journal.pone.0214829
    1. Starnes LM, Downey CM, Boyd SK, Jirik FR. Increased bone mass in male and female mice following tamoxifen administration. Genesis. 2007;45:229-235. doi:10.1002/dvg.20294
    1. Zhong ZA, Sun W, Chen H, et al. Optimizing tamoxifen-inducible Cre/loxp system to reduce tamoxifen effect on bone turnover in long bones of young mice. Bone. 2015;81:614-619. doi:10.1016/j.bone.2015.07.034

Publication types

LinkOut - more resources