Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Mar 10:10:tkac006.
doi: 10.1093/burnst/tkac006. eCollection 2022.

Applications of tetrahedral DNA nanostructures in wound repair and tissue regeneration

Yikai Dou  1 , Weitong Cui  2 , Xiao Yang  1 , Yunfeng Lin  2 , Xiaohong Ma  1 , Xiaoxiao Cai  2
Affiliations
Review

Applications of tetrahedral DNA nanostructures in wound repair and tissue regeneration

Yikai Dou et al. Burns Trauma. .

Abstract

Tetrahedral DNA nanostructures (TDNs) are molecules with a pyramidal structure formed by folding four single strands of DNA based on the principle of base pairing. Although DNA has polyanionic properties, the special spatial structure of TDNs allows them to penetrate the cell membrane without the aid of transfection agents in a caveolin-dependent manner and enables them to participate in the regulation of cellular processes without obvious toxic side effects. Because of their stable spatial structure, TDNs resist the limitations imposed by nuclease activity and innate immune responses to DNA. In addition, TDNs have good editability and biocompatibility, giving them great advantages for biomedical applications. Previous studies have found that TDNs have a variety of biological properties, including promoting cell migration, proliferation and differentiation, as well as having anti-inflammatory, antioxidant, anti-infective and immune regulation capabilities. Moreover, we confirmed that TDNs can promote the regeneration and repair of skin, blood vessels, muscles and bone tissues. Based on these findings, we believe that TDNs have broad prospects for application in wound repair and regeneration. This article reviews recent progress in TDN research and its applications.

Keywords: Injury regeneration; Injury repair; Tetrahedral DNA nanostructures; Tissue regeneration; Wound treatment.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Schematic representation of composites used for wound repair and tissue regeneration
Figure 2.
Figure 2.
Successful synthesis and characterization of tetrahedral DNA nanostructures (TDNs). (a) Schematic diagram of TDNs. (b) Confirmation of the successful synthesis of TDNs by 8% polyacrylamide gel electrophoresis. Lanes 1–5: S1, S2, S3, S4 and TDNs, showing the successful synthesis of TDNs. (c) Transmission electron microscope images of TDNs. (d) Zeta potential graph and typical size distribution graph of TDNs
Figure 3.
Figure 3.
Tetrahedral DNA nanostructures play a vital role in repair and regeneration in several tissues
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Liang L, Li J, Li Q, Huang Q, Shi J, Yan H, et al. . Single-Particle Tracking and Modulation of Cell Entry Pathways of a Tetrahedral DNA Nanostructure in Live Cells. Angew Chem Int Ed. 2014;53:7745–50. - PubMed
    1. Zhang M, Zhang X, Tian T, Zhang Q, Wen Y, Zhu J, et al. . Anti-inflammatory activity of curcumin-loaded tetrahedral framework nucleic acids on acute gouty arthritis. Bioactive materials. 2022;8:368–80. - PMC - PubMed
    1. Hu Y, Cecconello A, Idili A, Ricci F, Willner I. Triplex DNA Nanostructures: From Basic Properties to Applications. Angew Chem Int Ed. 2017;56:15210–33. - PubMed
    1. Zhang Q, Lin S, Wang L, Peng S, Lin Y. Tetrahedral framework nucleic acids act as antioxidants in acute kidney injury treatment. Chem Eng J. 2020;127426.
    1. Goodman RP, Schaap IA, Tardin CF, Erben CM, Berry RM, Schmidt CF, et al. . Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication. Science (New York, NY). 2005;310:1661–5. - PubMed