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. 2022 Mar;29(3):1394-1401.
doi: 10.1016/j.sjbs.2021.12.061. Epub 2022 Jan 3.

Evaluation of the antioxidative and genotoxic effects of sodium butyrate on breast cancer cells

Burcu Yuksel  1 , Asuman Deveci Ozkan  2 , Duygu Aydın  3 , Zeynep Betts  3
Affiliations

Evaluation of the antioxidative and genotoxic effects of sodium butyrate on breast cancer cells

Burcu Yuksel et al. Saudi J Biol Sci. 2022 Mar.

Abstract

Oncogenic stimulation shows a rise in reactive oxygen species (ROS), and ROS can eventually induce carcinogenesis by causing DNA damage. In this context, this study aims to evaluate some biochemical and genotoxic changes in the control of cell death caused by NaBu (Sodium butyrate). treatment in breast cancer cells. NaBu's impact on cell proliferation was determined via WST-1 assay. The lipid peroxidation (MDA), reduced glutathione (GSH), Nitric Oxide (NO), hydrogen peroxide (H2O2), and superoxide dismutase (SOD) enzyme levels were determined biochemically. NaBu-induced genotoxic damage was estimated via single-cell gel electrophoresis (SCGE). NaBu reduced cell viability and potentially induced GSH, but decreased SOD enzyme activity and the level of MDA and NO decreased also H2O2 decreased at different times and NaBu concentrations. Higher NaBu concentrations amplified DNA damage in MCF-7 cells compared to the control group. NaBu shows anticancer and genotoxic effects, especially through antioxidant enzymes, one of the oxidative stress parameters in breast cancer. However, the anticancer and genotoxic effects of NaBu is changed in the oxidative stress parameters with time and treatment concentration of NaBu in MCF-7 cells. Furthermore, his oxidative stress-dependent effect changes need to be clarified by further evaluation with molecular and more biochemical parameters.

Keywords: Breast cancer; Comet assay; Nitric oxide; Sodium butyrate.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
The effects of NaBu on the cell viability of MCF-7 cells were determined by WST-1 analysis for 24 and 48 h (**p < .001).
Fig. 2
Fig. 2
The amounts of (A) MDA, (B) H2O2, (C) SOD, (D) NO and (E) GSH at the end of 24 and 48 h in the control group and NaBu-treated MCF-7 cells. The group without NaBu was determined as the negative control and the changes in the determined parameter (**p < .001).
Fig. 3
Fig. 3
The images of NaBu induced genotoxic effect determined by comet assay in MCF-7 cells after 48 h. The images were shown in triplicate (n = 3).
See this image and copyright information in PMC

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