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. 2022 Mar 6:15:883-893.
doi: 10.2147/IDR.S350715. eCollection 2022.

Comparative Proteomic Analysis Reveals Antibacterial Mechanism of Patrinia scabiosaefolia Against Methicillin Resistant Staphylococcus epidermidis

Xin Liu  1 , Lili An  2 , Shuaijun Ren  2 , Yonghui Zhou  1 , Wei Peng  1
Affiliations

Comparative Proteomic Analysis Reveals Antibacterial Mechanism of Patrinia scabiosaefolia Against Methicillin Resistant Staphylococcus epidermidis

Xin Liu et al. Infect Drug Resist. .

Abstract

Purpose: As a kind of opportunist pathogen, Staphylococcus epidermidis (MRSE) can cause nosocomial infections and easily evolve into resistant bacteria. Among these, methicillin-resistant Staphylococcus epidermidis (MRSE) exhibit significantly higher rates. Our previous study showed that Patrinia scabiosaefolia (PS) possessed strong antibacterial activity against MRSE. However, the mechanism of PS against MRSE is not clear.

Methods: Here, a tandem mass tag-based (TMT) proteomic analysis was performed to elucidate the potential mechanism of PS against MRSE. We compared the differential expression proteins of MRSE under PS stress.

Results: Based on a fold change of >1.2 or < 1/1.2 (with p value set at <0.05), a total of 248 proteins (128 up-regulated proteins, 120 down-regulated proteins) were identified. Bioinformatic analysis showed that proteins including arginine deiminase (arcA), ornithine carbamoyltransferase (arcB) and carbamate kinase (arcC), serine-tRNA ligase (serS), phenylalanine-tRNA ligase beta and subunit (pheT), DltD (dlt), d-alanyl carrier protein (dlt), accumulation-associated protein (SasG), serine-aspartate repeat-containing protein C (SdrC) and hemin transport system permease protein HrtB (VraG) played important roles in mechanism of PS against MRSE.

Conclusion: In summary, these results indicated that arginine deiminase pathway (ADI) pathway, protein synthesis, cell wall synthesis, biofilm formation and uptake of iron were related to mechanisms of PS against MRSE. Our findings provide an insight into the the mechanism of PS against MRSE, and may be valuable in offering new targets to develop more anti-MRSE drugs.

Keywords: Patrinia scabiosaefolia; methicillin-resistant Staphylococcus epidermidis; proteomic.

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Conflict of interest statement

The authors declare no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Significantly differential proteins of MRSE in 2.5mg/mL PS stress using TMT-based quantitative proteomics. (A) The horizontal axis is the relative quantitative protein value after Log2 logarithm conversion, and the vertical axis is the difference significance test p-value value after -Log10 logarithm conversion. The red dots indicate up-regulated proteins, and blue dots indicate down-regulated proteins. (B) The number of DEPs. Q1 (<0.769) and Q2 (0.769–0.833) represent down-regulated proteins, and Q3 (1.2–1.3) and Q4 (>1.3) represent up-regulated proteins.
Figure 2
Figure 2
Continued.
Figure 2
Figure 2
Continued.
Figure 2
Figure 2
Go annotation and KEGG pathway of DEPs: gene ontology terms for subcellular location distribution. (A) cellular component (B) biological process (C) molecular function (D) main KEGG pathway.
Figure 3
Figure 3
String network of DEPs of MRSE in PS stress. Colored lines between the proteins indicate the various types of interaction evidence. Structure which is drawn in the protein nodes indicated the availability of 3D protein structure information. (A) String network of up-regulated proteins (B) String network of down-regulated proteins.
Figure 4
Figure 4
The mRNA levels of four genes were respectively analyzed by qPCR method in MRSE with and without PS.
See this image and copyright information in PMC

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