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. 1986 Aug;24(2):298-300.
doi: 10.1128/jcm.24.2.298-300.1986.

Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium

H Hariharan, B A Booth, T J Brickman, W C Katt, M Boesman-Finkelstein, R A Finkelstein

Competitive enzyme-linked immunosorbent assay for cholera-related enterotoxins in Salmonella typhimurium

H Hariharan et al. J Clin Microbiol. 1986 Aug.

Abstract

We report a rapid competitive enzyme-linked immunosorbent assay to screen Salmonella typhimurium strains for cholera-related enterotoxin antigens. Polymyxin B extracts of bacterial cells from syncase-glucose broth cultures of 7 of 15 strains gave positive results. The specificity of the test was confirmed with known heat-labile-enterotoxin-positive and -negative Escherichia coli strains which gave significantly different values.

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