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. 1986 Aug 21;92(1):103-7.
doi: 10.1016/0022-1759(86)90509-0.

Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes

T Hough, M B Singh, I J Smart, R B Knox

Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes

T Hough et al. J Immunol Methods. .

Abstract

We have developed an immunofluorescent screening method in which animal or plant cells are immobilized without fixation on polycarbonate (Nuclepore) membranes. The technique does not involve centrifugation, and virtually no cells are lost during processing. Direct microscopic analysis of antibody binding provides an extremely sensitive and reproducible screening system for hybridoma supernatants.

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