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Review
. 2022 Mar 14;13(1):119-125.
doi: 10.1515/bmc-2022-0009.

The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Antonella Gradogna  1 , José M Pardo  2 , Armando Carpaneto  1   3
Affiliations
Free article
Review

The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Antonella Gradogna et al. Biomol Concepts. .
Free article

Abstract

In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional characterization of nonelectrogenic potassium/proton vacuolar antiporters of the NHX (Na+/H+ exchanger) family. Moreover, we review the functional role of the tonoplast-specific phosphoinositide PI(3,5)P2, able to simultaneously inhibit the activity of NHXs and CLC-a transporters, whose coordinated action can play an important role in the water balance of plant cells.

Keywords: BCECF; NHX antiporters; fluorescence; patch-clamp; plant vacuole; proton.

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References

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