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. 2022 Sep;116(6):356-364.
doi: 10.1080/20477724.2022.2035624. Epub 2022 Mar 14.

Identification of potential vectors of Dirofilaria immitis and Brugia pahangi (Spirurida: Filariidae): First observation of infective third-stage larva of B. pahangi in Culex quinquefasciatus (Diptera: Culicidae)

Wei Yin Vinnie-Siow  1 , Van Lun Low  2 , Tiong Kai Tan  1 , Meng Li Wong  2 , Cherng Shii Leong  1 , Nazni Wasi Ahmad  3 , Yvonne Ai Lian Lim  1
Affiliations

Identification of potential vectors of Dirofilaria immitis and Brugia pahangi (Spirurida: Filariidae): First observation of infective third-stage larva of B. pahangi in Culex quinquefasciatus (Diptera: Culicidae)

Wei Yin Vinnie-Siow et al. Pathog Glob Health. 2022 Sep.

Abstract

Information on the mosquito species that transmit canine filariosis is scanty. Hence, an experimental study was conducted to identify the potential vectors responsible for the transmission of D. immitis Leidy and B. pahangi Buckley & Edeson. A total of 367 mosquitoes belonging to six species containing both laboratory and field strains (i.e. Aedes togoi Theobald, Aedes aegypti Linnaeus, Aedes albopictus Skuse, Culex quinquefasciatus Say, Culex vishnui Theobald and Anopheles dirus Peyton & Harrison) were used in this study. All mosquitoes were artificially fed on either D. immitis or B. pahangi microfilariae (mfs) infected blood by using the Hemotek™ membrane feeding system. Out of 367 mosquitoes, 228 (64.9%) were fully engorged. After feeding on D. immitis (20%) and B. pahangi (33%) mfs positive blood, the mortality rates for Cx. quinquefasciatus were found to be slightly lower than that of other species of mosquitoes. On the other hand, majority of An. dirus were found to be incapable to withstand the infection of mfs as the mortality rates were relatively high (D. immitis = 71.4%; B. pahangi = 100.0%). Brugia pahangi was detected in Ae. togoi and Cx. quinquefasciatus with infection rates of 50% and 25%, respectively. Aedes togoi was the only species infected with D. immitis with an infection rate of 69%. Our results showed that Ae. togoi was an excellent experimental vector for both D. immitis and B. pahangi. This study also documented the observation of B. pahangi, for the first time in the head region of Cx. quinquefasciatus under a laboratory setting.

Keywords: Brugia pahangi; Dirofilaria immitis; filaria; mosquitoes; vector.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Percentage of accumulative daily mortality of Ae. togoi after blood feeding on individuals with and without microfilariae of D. immitis and B. pahangi in the peripheral blood stream.
Figure 2.
Figure 2.
L3 larva of B. pahangi at the head region of Ae. togoi.
See this image and copyright information in PMC

References

    1. Cringoli G, Rinaldi L, and Veneziano V, et al. A prevalence survey and risk analysis of filariasis in dogs from the Mt. Vesuvius area of southern Italy. Vet. Parasitol. 2001;102(3):243–252. - PubMed
    1. Denham DA, and McGreevy PB.. Brugian filariasis: epidemiological and experimental studies. Adv. Parasitol. 1977;15:243–309. - PubMed
    1. Mak JW, Yen PKF, and Lim KC, et al. Zoonotic implications of cats and dogs in filarial transmission in Peninsular Malaysia. Trop.Geogr. Med. 1980;32:259–264. - PubMed
    1. Silaghi C, Beck R, Capelli G, et al. Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus. Parasit Vectors. 2017;10(1). DOI:10.1186/s13071-017-2015-x - DOI - PMC - PubMed
    1. Kini RG, Leena JB, and Shetty P, et al. Human dirofilariasis: an emerging zoonosis in India. J. Parasitic. Dis. 2015;39(2):349–354. - PMC - PubMed