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. 1978 Jul 12;43(1):53-6.
doi: 10.1007/BF00396478.

A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes

W M Howell, D A Black

A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes

W M Howell et al. Hum Genet. .

Abstract

A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.

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