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. 2022 Jan;10(4):565-572.
doi: 10.52547/rbmb.10.4.565.

Analysis of a Major Phenolic Glucoside and Biochemistry Compounds from Curculigo Orchioides Gaertn

Thanh Pham  1 , Nu Ngoc Doan Huyen Phan  1 , Van Giang Tran  1 , Thi Quynh Nguyen  1 , Minh Duc Tran  2 , Thanh Hung Nguyen  3
Affiliations

Analysis of a Major Phenolic Glucoside and Biochemistry Compounds from Curculigo Orchioides Gaertn

Thanh Pham et al. Rep Biochem Mol Biol. 2022 Jan.

Abstract

Background: Orcinol-β-D-glucoside, which is also known as orcinol glucoside, is a major phenolic glucoside compound in the rhizome of the Curculigo orchioides Gaertn. This compound has many medicinal properties such as being antioxidant, immunomodulatory, antiosteoporosis, stress relief, antidepressant, etc.

Methods: Determination of reducing sugar content by Bertrand's method, determination of lipid content by Soxhlet method, determination of vitamin C content by iodine titration, determination of enzyme activity catalase by titration with KMnO4. Quantification of Orcinol-β-D-glucoside was conducted by HPLC analysis.

Results: The Orcinol-β-D-glucoside of C. orchioides in Thuy Bang mountain was highest. Besides, the content of reducing sugars, vitamin C, enzyme catalase, and lipids of C. orchioides differed significantly among sites. In which, the reducing sugar and vitamin C of C. orchioides in Ngu Binh mountain was highest. Whereas, enzyme catalase was also highest in Thuy Bang mountain. However, the lipid content of C. orchioides was also highest in Huong Tho mountain.

Conclusion: The result will contribute to providing the scientific basis for the selection of breeding, planting, development of C. orchioides in Thua Thien Hue province, as well as other provinces in Vietnam and opening new research directions for applications in the future.

Keywords: Antioxidants; Hypoxidaceae; Medicinal Plants; Vietnam.

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Figures

Fig. 1
Fig. 1
The sample of Curculigo orchioides in different site of Thua Thien Hue province, Vietnam
Fig. 2
Fig. 2
The calibration curve of Orcinol - β - D - glucoside in HPLC fingerprinting: The signal E+7 means 107.
Fig. 3
Fig. 3
HPLC chromatogram of the dry rhizome of Curculigo orchioides Gaertn. extract visualized by A) CT-3, B) CT-2, C) CT-1, and D) CT-4. The vertical axis is intensity (mAU), the horizontal axis is retention time (min). The retention time of Orcinol-β-D-glucoside compound in 4 formulas are all around the 20th minute.
Fig. 4
Fig. 4
HPLC Fingerprint of 04 samples by A) Ngu Binh mountain (Hue city), B) Phu Bai mountain (Phu Bai town), C) Huong Tho mountain (Huong Tra district), D) Thuy Bang mountain (Huong Thuy district). The vertical axis is intensity (mAU), the horizontal axis is retention time (min). The retention time of Orcinol-β-D-glucoside compound in 4 samples are all around the 20th minute.
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