Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 17;12(1):4585.
doi: 10.1038/s41598-022-08681-4.

Origin and genetic analysis of stem rust resistance in wheat line Tr129

Jyoti Saini Sharma #  1 , Thomas G Fetch #  1 , Habibollah Ghazvini  2 , Matthew N Rouse  3 , Tatiana Danilova  4 , Bernd Friebe  5 , Colin W Hiebert  6
Affiliations

Origin and genetic analysis of stem rust resistance in wheat line Tr129

Jyoti Saini Sharma et al. Sci Rep. .

Abstract

Wheat line Tr129 is resistant to stem rust, caused by Puccinia graminis f. sp. tritici (Pgt). The resistance in Tr129 was reportedly derived from Aegilops triuncialis, but the origin and genetics of resistance have not been confirmed. Here, genomic in situ hybridization (GISH) showed that no Ae. triuncialis chromatin was present in Tr129. Genetic and phenotypic analysis was conducted on F2 and DH populations from the cross RL6071/Tr129. Seedlings were tested with six Pgt races and were genotyped using an Illumina iSelect 90 K SNP array and kompetitive allele specific PCR (KASP) markers. Mapping and phenotyping showed that Tr129 carried four stem rust resistance (Sr) genes on chromosome arms 2BL (Sr9b), 4AL (Sr7b), 6AS (Sr8a), and 6DS (SrTr129). SrTr129 co-segregated with markers for SrCad, however Tr129 has a unique haplotype suggesting the resistance could be new. Analysis of a RL6071/Peace population revealed that like SrTr129, SrCad is ineffective against three North American races. This new understanding of SrCad will guide its use in breeding. Tr129 and the DNA markers reported here are useful resources for improving stem rust resistance in cultivars.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Comparison of the SrCad genomic region in four mapping populations: LMPG-6S/Triumph 64 (a), RL6071/Tr129 (b), RL6071/Peace (c), and LMPG-6S/Norin 40 (d). Markers shown in dark blue font were mapped in more than one mapping population and resistance genes are shown in dark orange font. Mapping distances are in centimorgans (cM).
Figure 2
Figure 2
Comparison of Sr8a linkage maps from RL6071/Tr129 DH population from the present study (a) and the LMPG-6S/Harvest DH population from Hiebert et al. (b). Common markers between two linkage maps are in dark blue font, and purple font was used for the postulated gene. Distances between loci are in centimorgans (cM).
Figure 3
Figure 3
Linkage maps developed for Subset 2 DH lines. (a) Sr7b mapped at 63.1 cM on chromosome arm 4AL; (b) Sr9b mapped at 79.2 cM on chromosome arm 2BL. Kompetitive allele specific (KASP) PCR markers designated as kwh developed in the present study are shown on the linkage maps. Primer information is provided in supplementary table S7.
See this image and copyright information in PMC

References

    1. Pretorius, Z. A., Singh, R. P., Wagoire, W.W. & Payne, T. S. Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis.84, 203 (2000). - PubMed
    1. Jin Y. Races of Puccinia graminis identified in the United States during 2003. Plant Dis. 2005;89:1125–1127. doi: 10.1094/PD-89-1125. - DOI - PubMed
    1. Fetch T, et al. Virulence of Ug99 (race TTKSK) and race TRTTF on Canadian wheat cultivars. Can. J. Plant Sci. 2012;92:602.
    1. Olivera, P. D. et al. Races of Puccinia graminis f. sp. tritici with combined virulence to Sr13 and Sr9e in a field stem rust screening nursery in Ethiopia. Plant Dis. 96, 623–628 (2012). - PubMed
    1. Olivera, P. et al. Phenotypic and genotypic characterization of race TKTTF of Puccinia graminis f. sp. tritici that caused a wheat stem rust epidemic in southern Ethiopia in 2013–14. Phytopathology105, 917–928 (2015). - PubMed

Publication types

Supplementary concepts