Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun;58(3):203-213.
doi: 10.1007/s11262-022-01893-w. Epub 2022 Mar 17.

Comparative trachea transcriptome analysis in SPF broiler chickens infected with avian infectious bronchitis and avian influenza viruses

Seyed Hossein Zamzam  1 , Arash Ghalyanchilangeroudi  2 , Ali Reza Khosravi  1
Affiliations

Comparative trachea transcriptome analysis in SPF broiler chickens infected with avian infectious bronchitis and avian influenza viruses

Seyed Hossein Zamzam et al. Virus Genes. 2022 Jun.

Abstract

Infectious bronchitis virus (IBV) and avian influenza virus (AIV) are two major respiratory infections in chickens. The coinfection of these viruses can cause significant financial losses and severe complications in the poultry industry across the world. To examine transcriptome profile changes during the early stages of infection, differential transcriptional profiles in tracheal tissue of three infected groups (i.e., IBV, AIV, and coinfected) were compared with the control group. Specific-pathogen-free chickens were challenged with Iranian variant-2-like IBV (IS/1494), UT-Barin isolates of H9N2 (A/chicken/Mashhad/UT-Barin/2017), and IBV-AIV coinfection; then, RNA was extracted from tracheal tissue. The Illumina RNA-sequencing (RNA-seq) technique was employed to investigate changes in the Transcriptome. Up- and downregulated differentially expressed genes (DEGs) were detected in the trachea transcriptome of all groups. The Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology databases were examined to identify possible relationships between DEGs. In the experimental groups, upregulated genes were higher compared to downregulated genes. A more severe immune response was observed in the coinfected group; further, cytokine-cytokine receptor interaction, RIG-I-like receptor signaling, Toll-like receptor signaling, NOD-like receptor signaling, Janus kinase/signal transducer, and activator of transcription, and apoptotic pathways were important upregulated genes in this group. The findings of this paper may give a better understanding of transcriptome changes in the trachea during the early stages of infection with these viruses.

Keywords: Avian infectious bronchitis virus; Avian influenza virus; Coinfection; Transcriptome.

PubMed Disclaimer

References

    1. Cook JK, Jackwood M, Jones R (2012) The long view: 40 years of infectious bronchitis research. Avian Pathol 41(3):239–250 - DOI
    1. Hassan KE, Shany SA, Ali A, Dahshan A-HM, Azza A, El-Kady MF (2016) Prevalence of avian respiratory viruses in broiler flocks in Egypt. Poult Sci 95(6):1271–1280 - DOI
    1. Roussan D, Haddad R, Khawaldeh G (2008) Molecular survey of avian respiratory pathogens in commercial broiler chicken flocks with respiratory diseases in Jordan. Poult Sci 87(3):444–448 - DOI
    1. Seifi S, Asasi K, Mohammadi A (2012) An experimental study on broiler chicken co-infected with the specimens containing avian influenza (H9subtype) and infectious bronchitis (4/91 strain) viruses. Iran J Vet Res 13(2):39
    1. Fernandez-Siurob I, Retana M, Tellez G, Arroyo-Navarro L, Bañuelos-Hernandez B, Castellanos-Huerta I (2014) Assessment of viral interference using a chemical receptor blocker against avian influenza and establishment of protection levels in field outbreaks. Vaccine 32(11):1318–1322 - DOI

LinkOut - more resources