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. 2022 Apr 5;58(28):4484-4487.
doi: 10.1039/d2cc00488g.

A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

Dayong Li  1 , Chengjie Duan  1 , Wenting Cheng  1 , Youjing Gong  1 , Yanheng Yao  1 , Xiaoping Wang  2 , Zhongyun Wang  3 , Yang Xiang  1   4
Affiliations

A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

Dayong Li et al. Chem Commun (Camb). .

Abstract

A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.

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