Serum miR-497-5p serves as a diagnostic biomarker for acute coronary syndrome and predicts the occurrence of major adverse cardiovascular events after percutaneous coronary intervention

Abstract

This study aimed to investigate the diagnostic value of microRNA (miR)-497-5p in acute coronary syndrome (ACS) and its predictive value for the occurrence of adverse major adverse cardiovascular events (MACEs). Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression of serum miR-497-5p in 110 ACS patients and 82 controls. And miR-497-5p levels were found to be significantly elevated in the patients (P < 0.001). Pearson correlation coefficient confirmed that miR-497-5p was positively correlated with Gensini scores (r = 0.684). The area under the Receiver-operating characteristic (ROC) curve was 0.861, which significantly identified patients with ACS, and was confirmed by logistic regression (OR = 8.533, 95%CI = 4.113-17.787, P < 0.001). Kaplan-Meier and Cox regression was performed to evaluate the predictive value of miR-497-5p in the occurrence of MACEs during a 6-month follow-up after percutaneous coronary intervention (PCI) in patients with ACS. The results demonstrated that miR-497-5p was an independent predictor of MACEs (HR = 4.773, 95%CI = 1.569-12.036, P = 0.013) and that patients with high level of miR-497-5p were more likely to develop MACEs after PCI (long-rank P = 0.019). Finally, miR-497-5p positively correlated with endothelial proinflammatory and adhesion factors. Our study suggests that serum miR-497-5p is a potential diagnostic marker for ACS and its elevated levels can predict a high risk of MACEs in ACS patients after PCI. And this may be associated with vascular endothelial injury.

Keywords: acute coronary syndrome; clinical biomarker; miR-497-5p.

Graphical abstract

Figure 1.

The serum expression of miR-497-5p in acute coronary syndromes patients and the correlation with Gensini score. (a) Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to detect serum miR-497-5p levels in patients with acute coronary syndrome and healthy individuals. (b) In acute coronary syndromes patients, the Pearson correlation coefficient examined the relationship of miR-497-5p and Gensini score. ***P < 0.001 versus controls.

Figure 2.

Receiver-operating characteristic (ROC) curves verified the clinical diagnostic value of miR-497-5p in predicting ACS.

Figure 3.

Kaplan-Meier survival curves based on serum miR-497-5p expression in ACS patients at 6 months.

Figure 4.

Correlation between serum miR-497-5p and endothelial adhesion factors ICAM-1 (a) VCAM-1 (b) as well as inflammatory factors IL-1β (c) and TNF-α (d) was evaluated by Person correlation coefficient.