Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 18;17(3):e0265649.
doi: 10.1371/journal.pone.0265649. eCollection 2022.

Nanoencapsulation of buriti oil (Mauritia flexuosa L.f.) in porcine gelatin enhances the antioxidant potential and improves the effect on the antibiotic activity modulation

Neyna de Santos Morais  1 , Thaís Souza Passos  2 , Gabriela Rocha Ramos  3 , Victoria Azevedo Freire Ferreira  3 , Susana Margarida Gomes Moreira  4 , Gildácio Pereira Chaves Filho  4 , Ana Paula Gomes Barreto  3 , Pedro Ivo Palacio Leite  5 , Ray Silva de Almeida  5 , Cícera Laura Roque Paulo  5 , Rafael Fernandes  6 , Sebastião Ânderson Dantas da Silva  1 , Sara Sayonara da Cruz Nascimento  7 , Francisco Canindé de Sousa Júnior  1   3 , Cristiane Fernandes de Assis  1   3
Affiliations

Nanoencapsulation of buriti oil (Mauritia flexuosa L.f.) in porcine gelatin enhances the antioxidant potential and improves the effect on the antibiotic activity modulation

Neyna de Santos Morais et al. PLoS One. .

Abstract

The present study evaluated the cytotoxicity, antioxidant potential, and antimicrobial effect on the antibiotic activity modulation of gelatin nanoparticles containing buriti oil (OPG). The cytotoxicity analysis was performed on Chinese Hamster Ovary Cells (CHO) using a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test. The antioxidant potential of buriti oil and OPG was determined by total antioxidant capacity, reducing power, and the ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) test. The modulating antimicrobial activity was evaluated by determining the minimum inhibitory concentration (MIC) concentration against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, gentamicin and norflaxacillin. The nanoformulation of OPG did not show a cytotoxic effect on CHO cells and had a higher antioxidant potential than free buriti oil (p<0.05). The combination of antibiotics with free buriti oil and OPG was more efficient in inhibiting E. coli and P. aeruginosa than isolated norfloxacillin and gentamicin (p<0.05). Regarding the inhibition of S. aureus, OPG in combination with norfloxacillin reduced MIC by 50%. Nanoencapsulation was a viable alternative to enhance functionality and adding commercial value to buriti oil.

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Characterization of buriti oil nanoencapsulated in porcine gelatin.
A. Micrograph obtained by Scanning Electron Microscopy. B. Dynamic light scattering. C. Fourier Transform Infrared Spectroscopy of nanoencapsulated buriti oil (a), buriti oil (b), Tween 20 (c), porcine gelatin (d).
Fig 2
Fig 2. Zeta Potential of buriti oil nanoencapsulated in porcine gelatin under different pH conditions.
Fig 3
Fig 3. Thermogravimetry graphs and differential thermal analysis.
a) Porcine gelatin b) Tween 20 c) Buriti oil d) OPG.
Fig 4
Fig 4
Cell viability (%) through the MTT assay in CHO-KI cells evaluated at different times (A– 24h, B– 48h, and C– 72h) and concentrations of buriti oil and OPG.
Fig 5
Fig 5
Reducing power test of crude buriti oil (A) and OPG nanoformulation. The data obtained presented parametric distribution. Therefore, the ANOVA test with Tukey’s post-test was used to determine the significant differences. *Equal letters indicate that the values do not differ statistically (p > 0.05).
Fig 6
Fig 6
MIC values (μg.mL-1) of antibiotics in a bacterial growth assay in the presence of buriti oil (A) and buriti oil nanoencapsulated in porcine gelatin (OPG) (B). The tests were performed under the following conditions: Norfloxacillin (white bar), norfloxacillin + crude buriti oil/OPG (light gray bar), gentamicin (dark gray bar), and gentamicin + crude buriti oil/OPG (black bar). ***p<0.0001.
See this image and copyright information in PMC

References

    1. Cattani IM, Baruque-Ramos J. Brazilian buriti palm fiber (Mauritia flexuosa Mart.). In Natural fibres: Advances in science and technology towards industrial applications. Springer, Dordrecht: 2016; 89–98. Available from: 10.1007/978-94-017-7515-1. - DOI
    1. Cândido TLN, Silva MR, Agostini-Costa TS. Bioactive compounds and antioxidant capacity of buriti (Mauritia flexuosa L.f.) from the Cerrado and Amazon biomes. Food Chem 2015; 177: 313–319. Available from: doi: 10.1016/j.foodchem.2015.01.041 - DOI - PubMed
    1. Cândido TLN, Silva MR. Comparison of the physicochemical profiles of buriti from the Brazilian Cerrado and the Amazon region. Food Sci Technol 2017; 37: 78–82. Available from: 10.1590/1678-457X.32516. - DOI
    1. Silva SM, Sampaio KA, Taham T, et al.. Characterization of oil extracted from buriti fruit (Mauritia flexuosa) grown in the Brazilian Amazon Region. JAOCS, J Am Oil Chem Soc 2009; 86: 611–616. Available from: 10.1007/s11746-009-1400-9. - DOI
    1. Ferreira BS, De Almeida CG, Faza LP, et al.. Comparative properties of amazonian oils obtained by different extraction methods. Molecules 2011; 16: 5874–5885. Available from: doi: 10.3390/molecules16075875 - DOI - PMC - PubMed

Publication types