Comparison of micro-ID, API 20E, and conventional media systems in identification of Enterobacteriaceae

Abstract

The Micro-ID, a new identification kit for Enterobacteriaceae, consists of 15 biochemical tests, with substrates and reagents impregnated in filter paper disks. A 0.2-ml amount of an organism suspension equal to a 0.5 McFarland standard is pipetted into each of the compartments. After 4 h of incubation and addition of potassium-hydroxide (KOH) to the Voges-Proskauer test, the color reactions are read according to the recommendations of the manufacturer. A five-digit octal code number is derived from each set of reactions from which an identification is derived by using a code book. In a single-blind, comparative study of the Micro-ID system with the API 20E system (Analytab Products Inc.) and conventional biochemical tube media, we found that the Micro-ID and the API 20E systems gave a 90% identification correlation when each was compared with the conventional tube media. A comparison of all three systems gave an 82% overall identification correlation. When the common tests of Micro-ID and API 20E were compared with conventional tube media, we found that the tests in the Micro-ID performed as well as or better than those of the API 20E. Certain groups of organisms, i.e., Citrobacter, Enterobacter, Proteus, Salmonella, and Serratia genera, were found to give low correlation on certain common tests. When using primary isolation MacConkey plates from the clinical laboratory, only 74% of the plates with Enterobacteriaceae had sufficient numbers of colonies of each enteric organism to produce the 0.5 McFarland inoculum density required. Problems concerning the misidentification of some organisms are discussed.