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. 2022 Feb 15;12(1):28-34.
eCollection 2022.

Application of berberine-loaded albumin nanoparticles in infections of traumatic wounds

Zhenqiang Liu  1 , Yanchao Liu  2 , Ting Fang  3 , Jianhua Xia  4 , Ning Ma  5 , Yanhong Wang  1
Affiliations

Application of berberine-loaded albumin nanoparticles in infections of traumatic wounds

Zhenqiang Liu et al. Int J Burns Trauma. .

Abstract

In recent years, the morbidity of infections in traumatic wounds has been on the increase. There are not many kinds of drugs for clinical treatment of infections, and their efficacy and safety are limited. Plant antimicrobial drugs are increasingly popular in mainstream medicine due to the challenges of traditional antibiotics abuse. Berberine has a scavenging effect on infections, however, berberine was restricted from using as a drug preparation with poor stability and bioavailability. Due to the low toxicity of nanoparticles, the green-synthetic, size-controlled approach of nanoparticles has been paid more attention. Therefore, based on the intermolecular disulfide bond network platform built earlier, we designed and developed a strategy to assemble molecular bovine serum albumin into large-sized nanostructures through the reconstructed intermolecular disulfide bond and hydrophobic interaction, and berberine with poor water solubility was encapsulated in it. Nanoassembly with bovine serum albumin increased biostability of berberine and significantly improved its activity against Staphylococcus Aureus (S.aureus) activity, which gives some new insights into the preparation and development of anti-infectives for Chinese medicine.

Keywords: Infections; albumin nanoparticles; berberine; green-synthetic.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Preparation and characterization of BBR-NP. A. Particle size of BBR-NP (the proportion between BBR and BSA is w/w=1:5); B. Particle size of BBR-NP (w/w=1:10); C. The TEM image of BBR-NP; D. The zeta potential of BBR-NP (w/w=1:10).
Figure 2
Figure 2
Studies on UV-vis spectroscopy. A. The absorption peak of BBR; B. The standard curve of BBR at 345 nm.
Figure 3
Figure 3
Studies on UV-vis of BBR-NP and release. A. The absorption peak of BBR, BBR-NP, BSA; B. The percentage of in vitro BBR released from BBR-NP in pH 5.5 and 7.4 PBS buffer.
Figure 4
Figure 4
The stability and stabilizing forces of BBR-NP. A. Long-term stability analysis of BBR-NP by size change determination for 60 days; B. Stability analysis of BBR-NP in 10% FBS (***P<0.001).
Figure 5
Figure 5
Effect of SDS, DTT and Urea on the size of BBR-NP. 1 to 7 are Control, SDS, DTT, Urea, SDS+DTT, SDS+Urea, SDS+DTT+Urea, respectively (***P<0.001).
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